Western Blot staining of HEK293 lysate overexpressing Human MGAT1 (c-term Myc tagged and loaded in Lane A and C) stained with PA5-19371 at a concentration of 1 ug/ml (lane A). Lane C was stained with anti-MYC Tag at a dilution of (1/1000). Lane B was loaded with mock-transfected HEK293 cells and stained using PA5-19371 at a concentration of 1 mg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (QVEKVRTNDR) corresponding to the internal amino acids of MGAT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, mouse and rat based on sequence homology.
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.