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Invitrogen
MGST1 Polyclonal Antibody
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FIGURE: 1 / 5
MGST1 Antibody (PA5-116438) in ICC/IF
Immunocytochemical analysis of MGST1 in hepG2 cells using a MGST1 polyclonal antibody (Product #PA5-116438). Formalin fixed cells, as seen in green, were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Product Details
PA5-116438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human MGST1 aa 1-144.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive controls: Siha cell lysate, HepG2 cell lysate, A549 cell lysate, mouse stomach tissue lysate, HepG2, siha, Rat Adrenal gland tissue, Human liver cancer tissue.
Target Information
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutathione S-transferase; glutathione S-transferase 12; MGC14525; MGST1; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I; peritoneal metastasis associated long noncoding RNA; unnamed protein product
Gene Aliases: 1500002K10Rik; Gst; GST12; MGST; MGST-I; MGST1; PMAN
UniProt ID: (Human) P10620, (Mouse) Q91VS7, (Rat) P08011
Entrez Gene ID: (Human) 4257, (Mouse) 56615, (Rat) 171341
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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