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Invitrogen
CXCR4 Monoclonal Antibody (12G5), PE
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FIGURE: 1 / 6
CXCR4 Antibody (MHCXCR404) in Flow
The Journal of pathology 2023 -
2 Figure KRT19 is dispensable for PP and PDLO formation. (A) Scheme of KRT19 knockout (KO) cell line generation and differentiation to PPs and PDLOs. (B) Flow cytometry of respective differentiation markers of WT and KRT19 (KO) cell lines at DE (stage markers CXCR4 and KIT), PE (stage marker PDX1), and PP (markers NKX6-1 and PDX1) stage of differentiation. N = 3 independent differentiations with two cell li... View More
Product Details
MHCXCR404
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
12G5
Immunogen
SIV-infected SupT1 cells injected into Balb/c mice
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Analyte Specific Reagent
How to use the Panel Builder
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Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; fusin; G protein-coupled receptor; HUMSTR; LESTR; G protein-coupled receptor; G-protein coupled receptor; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; unnamed protein product
Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS; WHIMS1
UniProt ID: (Human) P61073
Entrez Gene ID: (Human) 7852
virus receptor activity
actin binding
G-protein coupled receptor activity
chemokine receptor activity
protein binding
coreceptor activity
C-C chemokine receptor activity
C-X-C chemokine receptor activity
cytokine binding
C-C chemokine binding
ubiquitin protein ligase binding
myosin light chain binding
C-X-C motif chemokine 12 receptor activity
ubiquitin binding
response to hypoxia
dendritic cell chemotaxis
apoptotic process
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
brain development
response to virus
cell migration
calcium-mediated signaling
neurogenesis
regulation of cell adhesion
regulation of cell migration
positive regulation of cell migration
CXCL12-activated CXCR4 signaling pathway
myelin maintenance
viral entry into host cell
positive regulation of oligodendrocyte differentiation
cell chemotaxis
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
positive regulation of cold-induced thermogenesis
positive regulation of vasculature development
positive regulation of macrophage migration inhibitory factor signaling pathway
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