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Invitrogen
Zap-70 Monoclonal Antibody (1E7.2), Pacific Blue™
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Product Details
MHZAP7028
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1E7.2
Immunogen
GST-fusion protein of Human ZAP70, amino acids 282-307
Conjugate
Excitation/Emission Max
404/455 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS with 4mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Analyte Specific Reagent
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Bioinformatics
Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; EC 2.7.10.2; FLJ17670; FLJ17679; kinase ZAP70; Selective T cell defect; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; unnamed protein product; ZA70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Gene Aliases: ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70
UniProt ID: (Human) P43403, (Mouse) P43404
Entrez Gene ID: (Human) 7535, (Mouse) 22637
nucleotide binding
phosphotyrosine binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
receptor binding
transferase activity, transferring phosphorus-containing groups
immune system process
protein phosphorylation
immune response
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
cell migration
peptidyl-tyrosine phosphorylation
cell differentiation
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
beta selection
positive thymic T cell selection
negative thymic T cell selection
thymic T cell selection
innate immune response
positive regulation of T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
protein autophosphorylation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
adaptive immune response
leukocyte cell-cell adhesion
calcium-mediated signaling
T cell differentiation
T cell activation
B cell activation
positive regulation of MAPK cascade
alpha-beta T cell differentiation
leukocyte migration
T cell aggregation
T cell migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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