Immunofluorescent analysis of MIF in U2OS cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a MIF mouse monoclonal antibody (Product # 367401) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® Plus 488 conjugate (Product # ) at a dilution of 1:500 for at least 30 minutes at room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat MIF fusion protein|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2,000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
36-7401 detects MIF from human, mouse and rat samples.
36-7401 has been successfully used in Western blot, immunohistochemical, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing MIF. Immunofluorescent staining in U2OS cells and Immunohistochemical staining of MIF in rat epididymis tissue with 36-7401. yields a staining pattern consistent with MIF in nucleus and cytosol.
MIF (Macrophage Migration Inhibitory Factor) is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through suppression of the anti-inflammatory effects of glucocorticoids. MIF may also play a role in integrin signaling pathways, because it has been found to form a complex in the cytosol near the peripheral plasma membrane with the JAB1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway.
36-7401 was used in immunocytochemistry to evaluate a novel nanotube in the promotion of insulin production from pancreatic beta cells
|Ilie I,Ilie R,Mocan T,Tabaran F,Iancu C,Mocan L||International journal of nanomedicine (8:3345)||2013|
|Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear.||Bank LM,Bianchi LM,Ebisu F,Lerman-Sinkoff D,Smiley EC,Shen YC,Ramamurthy P,Thompson DL,Roth TM,Beck CR,Flynn M,Teller RS,Feng L,Llewellyn GN,Holmes B,Sharples C,Coutinho-Budd J,Linn SA,Chervenak AP,Dolan DF,Benson J,Kanicki A,Martin CA,Altschuler R,Koch AE,Koch AE,Jewett EM,Germiller JA,Barald KF||Development (Cambridge, England) (139:4666)||2012|
|Mouse||Not Cited||Ribosomal protein S19 interacts with macrophage migration inhibitory factor and attenuates its pro-inflammatory function.||Filip AM,Klug J,Cayli S,Fröhlich S,Henke T,Lacher P,Eickhoff R,Bulau P,Linder M,Carlsson-Skwirut C,Leng L,Bucala R,Kraemer S,Bernhagen J,Meinhardt A||The Journal of biological chemistry (284:7977)||2009|
||The role of macrophage migration inhibitory factor in ocular surface disease pathogenesis after chemical burn in the murine eye.||Oh SY,Choi JS,Kim EJ,Chuck RS,Park CY||Molecular vision (16:2402)||2010|
|Mouse||1:200||The role of macrophage migration inhibitory factor in ocular surface disease pathogenesis after chemical burn in the murine eye.||Oh SY,Choi JS,Kim EJ,Chuck RS,Park CY||Molecular vision (16:2402)||2010|