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Invitrogen
MIF Polyclonal Antibody
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FIGURE: 1 / 9
MIF Antibody (36-7401) in ICC/IF
Immunofluorescent analysis of MIF in U2OS cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a MIF mouse monoclonal antibody (Product # 36-7401) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
36-7401
Applications
Tested Dilution
Publications
Product Specifications
Published species
Chicken,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant rat MIF fusion protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Product Specific Information
36-7401 detects MIF from human, mouse and rat samples.
36-7401 has been successfully used in Western blot, immunohistochemical, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing MIF. Immunofluorescent staining in U2OS cells and Immunohistochemical staining of MIF in rat epididymis tissue with 36-7401. yields a staining pattern consistent with MIF in nucleus and cytosol.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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