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Invitrogen
MINT3 Polyclonal Antibody
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FIGURE: 1 / 4
MINT3 Antibody (PA1-072) in ICC/IF
Immunofluorescence analysis of Mint3 was performed using 90% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Mint3 Rabbit Polyclonal Antibody (Product # PA1-072) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA1-072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) E F L P E P Q H P P G P P T M D L E(19) of rat Mint3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-072 detects munc-18 interacting protein 3 (Mint3) from mouse cells and rat brain samples.
PA1-072 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~86 kDa protein representing Mint3 from AtT20 cell extract and rat brain samples.
The PA1-072 immunogen is a synthetic peptide corresponding to residues M(1) E F L P E P Q H P P G P P T M D L E(19) of rat Mint3. The PA1-072 immunizing peptide (Cat. # PEP-135) is available for use in neutralization and control experiments.
Target Information
The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimer's Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adapter protein X11gamma; amyloid beta (A4) precursor protein-binding family A member 3 (X11-like 2); amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2); amyloid beta (A4) precursor protein-binding, family A, member 3 (X11-like 2); amyloid beta A4 precursor protein-binding family A member 3; Amyloid-beta A4 precursor protein-binding family A member 3; lin 10; Mint 3; Mint-3; Neuron-specific X11L2 protein; Neuronal Munc18-1-interacting protein 3; phosphotyrosine-binding/-interacting domain (PTB)-bearing protein; X11-like 2 protein
Gene Aliases: APBA3; AW208791; lin-10; MGC:15815; Mint-3; MINT3; X11gamma; X11L2
UniProt ID: (Human) O96018, (Rat) O70248, (Mouse) O88888
Entrez Gene ID: (Human) 9546, (Rat) 83611, (Mouse) 57267
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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