|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Tested Species reactivity||Hamster, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length protein.|
|Storage conditions||4° C|
MA1-25669 detects MLH1 from human, rat, and hamster samples.
MA1-25669 has been successfully used in immunohistochemistry (paraffin) procedures.Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested positive controls for this product are tonsil and colon carcinoma.
The MA1-25669 immunogen is recombinant, full length protein.
MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: COCA2; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1-like protein; FCC2; hMLH1; HNPCC; HNPCC2; MGC5172; mismatch repair protein 1; mutL homolog 1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1
Gene Aliases: COCA2; FCC2; hMLH1; HNPCC; HNPCC2; I79_014127; MLH1
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