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Bethyl Laboratories
MLK4 Polyclonal Antibody
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FIGURE: 1 / 1
MLK4 Antibody (A302-609A) in IP
Detection of human MLK4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLK4 antibody (Product # A302-609A) used for IP at 3 µg/mg lysate. MLK4 was also immunoprecipitated by rabbit anti-MLK4 antibody A302-610A, which recognizes a downstream epitope. For blotting immunoprecipitated MLK4, A302-610A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Product Details
A302-609A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 650 and 700 of human mixed lineage kinase 4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4 and does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitogen-activated protein kinase kinase kinase 21; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK4
Gene Aliases: dJ862P8.3; KIAA1804; MAP3K21; MLK4
UniProt ID: (Human) Q5TCX8
Entrez Gene ID: (Human) 84451
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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