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Invitrogen
IL-12 p70 Monoclonal Antibody (C17-8), Biotin
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Product Details
MM121B
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
C17-8
Immunogen
Recombinant mouse IL-12
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
250 µg
Purification
Protein G
Storage buffer
PBS with 4% BSA
Contains
proprietary Preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MM121B targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM121B immunogen is recombinant mouse IL-12.
MM121B detects IL-12 which has a predicted molecular weight of approximately 22 kDa.
The MM121B mouse IL12 antibody (biotinylated conjugate of clone C17-8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM120 (clone 9A5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody MM121B (clone C17-8) and antibody MM120 (clone 9A5) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
Target Information
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 35 kDa subunit; cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p35 subunit; IL-12 p40 subunit; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-23 subunit p40; IL12A/B; IL12AB; ILN; Interleukin; interleukin 12a p35 subunit; interleukin-12 p35 subunit; interleukin-12 p40 subunit; Interleukin-12 p70; MGC151228; MGC151232; unnamed protein product
Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; Il12p40; Ll12a; p35; p40
UniProt ID: (Mouse) P43431, (Mouse) P43432
Entrez Gene ID: (Mouse) 16159, (Mouse) 16160
cytokine activity
interleukin-12 receptor binding
protein binding
growth factor activity
interleukin-12 beta subunit binding
interleukin-27 binding
protein heterodimerization activity
cytokine receptor activity
cytokine receptor binding
interleukin-12 alpha subunit binding
identical protein binding
macromolecular complex binding
interleukin-23 receptor binding
positive regulation of leukocyte mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
natural killer cell activation involved in immune response
positive regulation of lymphocyte mediated immunity
positive regulation of T-helper 1 type immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
negative regulation of inflammatory response to antigenic stimulus
cell surface receptor signaling pathway
JAK-STAT cascade
cell proliferation
response to UV-B
cell migration
natural killer cell activation
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of mononuclear cell proliferation
positive regulation of smooth muscle cell apoptotic process
interleukin-12-mediated signaling pathway
interleukin-23-mediated signaling pathway
T-helper cell differentiation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
defense response to protozoan
T-helper 1 cell differentiation
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
positive regulation of JAK-STAT cascade
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
negative regulation of protein secretion
regulation of inflammatory response
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
cellular response to lipopolysaccharide
cellular response to interferon-gamma
negative regulation of vascular endothelial growth factor signaling pathway
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
positive regulation of T-helper 17 type immune response
immune response
response to virus
response to lipopolysaccharide
T-helper 1 cell activation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of T cell differentiation
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
extrinsic apoptotic signaling pathway
cellular response to virus
positive regulation of dendritic cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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