Invitrogen

MMP13 Polyclonal Antibody

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MMP13 Antibody in Western Blot (WB) — Western blot analysis of MMP13 in Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with MMP13 polyclonal antibody (Product # PA5-95401) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Product Details

Tested Applications

Dilution

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Non-human primate

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MMP13, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 0.9mg NaCl

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Synthetic peptide sequence: 109-154aa, RTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFT.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

MMP13 (collagenase 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves triple helical collagens, including type I, II, and III collagen-but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossification. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3.4.24.-; Collagenase 3; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP-13; MMP13

Gene Aliases: CLG3; MANDP1; MMP-13; MMP13

UniProt ID: (Human) P45452

Entrez Gene ID: (Human) 4322

Function(s)

luteolysis osteoblast differentiation response to hypoxia endochondral ossification growth plate cartilage development proteolysis heart development parturition response to mechanical stimulus extracellular matrix disassembly bone mineralization collagen catabolic process embryonic hindlimb morphogenesis response to drug peptide catabolic process response to estrogen cellular protein metabolic process estrous cycle bone morphogenesis cellular response to fluid shear stress positive regulation of pancreatic trypsinogen secretion

Process(es)

fibronectin binding metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding collagen binding zinc ion binding calcium-dependent protein binding low-density lipoprotein particle receptor binding

MMP13 Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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