Proteintech

MRPL23 Polyclonal Antibody

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MRPL23 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

MRPL23 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Product Details

11706-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPL23 Fusion Protein Ag2220 (1-153 aa encoded by BC027710) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MARNVVYPL YRLGGPQLRV FRTNFFIQLV RPGVAQPEDT VQFRIPMEMT RVDLRNYLEG IYNVPVAAVR TRVQHGSNKR RDHRNVRIKK PDYKVAYVQL AHGQTFTFPD LFPEKDESPE GSAADDLYSM LEEERQQRQS SDPRRGGVPS WFGL (1-153 aa encoded by BC027710)

Target Information

MRPL23 are mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL23 encodes a 39S subunit protein and is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A variant in MRPS14 (uS14m) causes perinatal hypertrophic cardiomyopathy with neonatal lactic acidosis, growth retardation, dysmorphic features and neurological involvement.

Human molecular genetics

Jackson CB,Huemer M,Bolognini R,Martin F,Szinnai G,Donner BC,Richter U,Battersby BJ,Nuoffer JM,Suomalainen A,Schaller A

Fri Feb 15 00:00:00 EST 2019

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Nature communications

Richman TR,Spåhr H,Ermer JA,Davies SM,Viola HM,Bates KA,Papadimitriou J,Hool LC,Rodger J,Larsson NG,Rackham O,Filipovska A

Mon Jun 20 00:00:00 EDT 2016

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

The Journal of cell biology

Richter U,Lahtinen T,Marttinen P,Suomi F,Battersby BJ

Mon Oct 26 00:00:00 EDT 2015

Mutation in MRPS34 compromises protein synthesis and causes mitochondrial dysfunction.

PLoS genetics

Richman TR,Ermer JA,Davies SM,Perks KL,Viola HM,Shearwood AM,Hool LC,Rackham O,Filipovska A

Sun Mar 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: 39S ribosomal protein L23, mitochondrial; FLJ45387; L23 mitochondrial-related protein; L23mt; Mitochondrial large ribosomal subunit protein uL23m; MRP-L23; Ribosomal protein L23-like; ribosomal protein related to L23 (mitochondrial)

Gene Aliases: L23MRP; MRPL23; RPL23; RPL23L

UniProt ID: (Human) Q16540

Entrez Gene ID: (Human) 6150

Molecular Function: ribosomal protein