Western blot analysis of DANRE stk3 in huaman placenta and zebra fish brain tissue lysate using 35 µg of protein per lane. Samples were probed with DANRE stk3 polyclonal antibody (Product # PA5-72649) with a dilution of 1:1000, and a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:5000.

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Product Details

PA5-72649

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 228-252 amino acids from the Central region of zebrafish MST2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2718503

Target Information

STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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MST2 Polyclonal Antibody

MST2 Antibody (PA5-72649) in WB