|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the internal region of human MUC13|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Mucin 13 is a 512 amino acid hemopoietic transmembrane mucin belonging to a family of secreted and cell surface glycoproteins and may play a role in cell signaling. It contains a serine/threonine-rich mucin repeat domain predicted to be heavily O-glycosylated, three cysteine-rich EGF-like domains, a SEA domain and also has sites for N-glycosylation and phosphorylation. Activation of NF-kappa-B signaling pathway by the inflammatory cytokines TNF-alpha and TGF-beta leads to upregulation of Mucin 13. It is a homodimer of beta subunits and is highly expressed in epithelial tissues, gastrointestinal and respiratory tracts, kidney, small intestine, appendix and stomach. RT-PCR and immunoblot analysis supports the up regulation of Mucin 13 in gastric cancers and is usually present on the apical side of tubular glands in intestinal-type cancers and in the cytoplasm in diffuse cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.