Invitrogen

MYBPC3 Polyclonal Antibody

1 Published Figure

2 References

View all (54) MYBPC3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (1)

MYBPC3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

MYBPC3 Antibody (PA5-71701) in IHC (P)

Immunohistochemistry analysis of MYBPC3 in paraformaldehyde-fixed, paraffin-embedded human skeletal muscle tissue sections. Samples were incubated with MYBPC3 polyclonal antibody (Product # PA5-71701) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen... View More

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Product Details

PA5-71701

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2717555

Target Information

Myosin binding protein C, cardiac-type (MYBPC3) is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments. It modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Mutations affecting the gene can result in cardiomyopathy - familial hypertrophic 4, cardiomyopathy - dilated 1MM, or left ventricular non-compaction 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ca2+/calmodulin-dependent protein kinase II and protein kinase G oxidation contributes to impaired sarcomeric proteins in hypertrophy model.

ESC heart failure

Gömöri K,Herwig M,Budde H,Hassoun R,Mostafi N,Zhazykbayeva S,Sieme M,Modi S,Szabados T,Pipis J,Farkas-Morvay N,Leprán I,Ágoston G,Baczkó I,Kovács Á,Mügge A,Ferdinandy P,Görbe A,Bencsik P,Hamdani N

PA5-71701 was used in Immunohistochemistry (Paraffin), Western Blot to show VO-induced cardiomyocyte dysfunction via deranged phosphorylation of myofilament proteins and signalling pathways due to increased oxidative state of CaMKII and PKG and this might contribute to the development of hypertrophy.

Mon Aug 01 00:00:00 UTC 2022

De Novo Missense Mutations in TNNC1 and TNNI3 Causing Severe Infantile Cardiomyopathy Affect Myofilament Structure and Function and Are Modulated by Troponin Targeting Agents.

International journal of molecular sciences

Hassoun R,Budde H,Mannherz HG,Lódi M,Fujita-Becker S,Laser KT,Gärtner A,Klingel K,Möhner D,Stehle R,Sultana I,Schaaf T,Majchrzak M,Krause V,Herrmann C,Nowaczyk MM,Mügge A,Pfitzer G,Schröder RR,Hamdani N,Milting H,Jaquet K,Cimiotti D

PA5-71701 was used in Western Blotting to investigate the interaction of troponin variants, TNNC1 and TNNCI3, with actin and their inter-subunit interactions, as well as the structural integrity of reconstituted thin filaments.

Mon Sep 06 00:00:00 UTC 2021

De Novo Missense Mutations in TNNC1 and TNNI3 Causing Severe Infantile Cardiomyopathy Affect Myofilament Structure and Function and Are Modulated by Troponin Targeting Agents.

International journal of molecular sciences

Mon Sep 06 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: C-protein, cardiac muscle isoform; cardiac C-protein; Cardiac MyBP-C; DKFZp779E1762; Myosin-binding protein C, cardiac-type; truncated cardiac myosin-binding protein C

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Gene Aliases: CMD1MM; CMH4; FHC; LVNC10; MYBP-C; MYBPC3

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UniProt ID: (Human) Q14896, (Rat) P56741

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Entrez Gene ID: (Human) 4607, (Rat) 295929, (Mouse) 17868

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Function(s)

ATPase activator activity actin binding protein binding structural constituent of muscle myosin binding titin binding myosin heavy chain binding identical protein binding metal ion binding molecular_function structural constituent of cytoskeleton

Process(es)

regulation of heart rate heart morphogenesis muscle contraction regulation of striated muscle contraction cell adhesion regulation of heart contraction myosin filament assembly sarcomere organization ventricular cardiac muscle tissue morphogenesis cardiac muscle contraction muscle filament sliding positive regulation of ATPase activity regulation of muscle filament sliding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYBPC3 Polyclonal Antibody

MYBPC3 Antibody (PA5-71701) in IHC (P)

Product Details

PA5-71701

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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