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MYLK2 Antibody (PA5-89733) in WB
Western blot analysis of MYLK2 in extracts of Jurkat cells. Samples were incubated with MYLK2 Polyclonal antibody (Product # PA5-89733) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89733
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human MYLK2 (NP_1491091)
Conjugate
Form
Liquid
Concentration
1.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805751
Product Specific Information
Immunogen sequence: MATENGAVEL GIQNPSTDKA PKGPTGERPL AAGKDPGPPD PKKAPDPPTL KKDAKAPASE KGDGTLAQPS TSSQGPKGEG DRGGGPAEGS; Positive Samples: Jurkat; Cellular Location: Cytoplasm
Target Information
MYLK2 (skMLCK) is a calcium/calmodulin-dependent serine/threonine kinase expressed exclusively in adult skeletal muscle. MYLK2 encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MLCK2; myosin light chain kinase 2, skeletal muscle; Myosin light chain kinase 2, skeletal/cardiac muscle
Gene Aliases: 9830004H17Rik; BB138278; KMLC; MLCK; MLCK2; MYLK2; skMLCK
UniProt ID: (Human) Q9H1R3, (Mouse) Q8VCR8
Entrez Gene ID: (Human) 85366, (Mouse) 228785
calmodulin-dependent protein kinase activity
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
myosin light chain binding
non-receptor serine/threonine protein kinase
striated muscle contraction
neuromuscular synaptic transmission
positive regulation of gene expression
skeletal muscle satellite cell differentiation
peptidyl-threonine phosphorylation
regulation of muscle filament sliding
skeletal muscle cell differentiation
protein autophosphorylation
cardiac muscle tissue morphogenesis
cardiac muscle contraction
protein phosphorylation
water transport
regulation of muscle contraction
phosphorylation
positive regulation of fast-twitch skeletal muscle fiber contraction
regulation of MAPK cascade
regulation of neuronal synaptic plasticity
synaptic vesicle transport
regulation of cell cycle
cardiac muscle cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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