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MYSM1 Antibody (PA5-102696) in ICC/IF
Immunofluorescent analysis of MYSM1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MYSM1 polyclonal antibody (Product # PA5-102696) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102696
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYSM1(Accession Q5VVJ2), corresponding to amino acid residues A645-Y695.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852090
Product Specific Information
Antibody detects endogenous levels of total MYSM1.
Target Information
Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2A-DUB; Deubiquitinase MYSM1; histone H2A deubiquitinase MYSM1; Myb-like, SWIRM and MPN domain-containing protein 1; Myb-like, SWIRM and MPN domains 1; RP4-592A1.1
Gene Aliases: 2A-DUB; 2ADUB; C130067A03Rik; C530050H10Rik; KIAA1915; MYSM1; RGD1311787
UniProt ID: (Human) Q5VVJ2, (Mouse) Q69Z66
Entrez Gene ID: (Human) 114803, (Rat) 298247, (Mouse) 320713
DNA binding
transcription coactivator activity
thiol-dependent ubiquitin-specific protease activity
metallopeptidase activity
protein complex binding
histone binding
metal ion binding
molecular_function
chromatin binding
peptidase activity
hydrolase activity
pyroglutamyl-peptidase activity
chromatin/chromatin-binding, or -regulatory protein
chromatin remodeling
transcription, DNA-templated
regulation of cell migration
monoubiquitinated histone H2A deubiquitination
positive regulation of transcription from RNA polymerase II promoter
regulation of hair follicle development
proteolysis
biological_process
regulation of transcription, DNA-templated
chromatin modification
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