|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Mixture of synthetic peptides EKSEDQDLQGLKDKP and APGTGRGRGRPKGSG, corresponding to aminoacids 11-25 and 181-195, respectively (Human).|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30643 detects MeCP2 from human samples.
PA1-30643 has been successfully used in Western blot applications.
The PA1-30643 immunogen is a mixture of synthetic peptides EKSEDQDLQGLKDKP and APGTGRGRGRPKGSG, corresponding to aminoacids 11-25 and 181-195, respectively (Human).
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.