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Invitrogen
Metallothionein Polyclonal Antibody
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Metallothionein Antibody (PA5-77772) in WB
Western blot analysis of Metallothionein in pseudomonas aeruginosa purified protein using 25 µg of protein. Sample was prepared with 1% milk, 2% BSA, incubated with Metallothionein polyclonal antibody (Product # PA5-77772) using a dilution of 1:1000 (16 hours at 4°C) and followed by a Mouse Anti-Rabbit secondary antibody at a dilution of 1:1000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: Blank, Lane 3: Pseudomonas aeruginosa purified protein with GST tag.
Product Details
PA5-77772
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of PA5-77772 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 36 kDa.
Target Information
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CES 1; CES1; Metallothionein; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT2; MT2A; Probable pmtA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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