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Midkine Antibody (500-P171BT-25UG) in WB
Western Blot: To detect hMidkine by Western Blot analysis Midkine Polyclonal Antibody, Biotin (Product # 500-P171BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMidkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P171BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Midkine
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929620
Product Specific Information
AA Sequence of recombinant protein: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Midkine. Anti-Human Midkine-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Midkine (500-P171) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMidkine.
Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMidkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P171BT-1MG will be provided as 2 x 500 µg
Target Information
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiregulin-associated protein; ARAP; FLJ27379; Midgestation and kidney protein; Midkine; MK; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; retinoic acid inducible factor
Gene Aliases: ARAP; MDK; MK; MK1; NEGF2
UniProt ID: (Human) P21741
Entrez Gene ID: (Human) 4192
behavioral fear response
signal transduction
Notch signaling pathway
nervous system development
short-term memory
response to wounding
cell migration
dentate gyrus development
cerebellar granular layer development
cerebral cortex development
cell differentiation
adrenal gland development
defecation
response to drug
negative regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
regulation of behavior
response to glucocorticoid
positive regulation of cell division
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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