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Invitrogen

Mitochondria Membrane Integrity Antibody Cocktail

2 Published Figures
1 Reference

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Product Details

45-7799

Applications
Tested Dilution
Publications

Western Blot (WB)

10.2 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.37 mg/mL

Amount

255 µg

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533833

Product Specific Information

Mitochondria Membrane Integrity cocktail of monoclonal antibodies specific to human, bovine, mouse, rat mitochondria membranes. This antibody is validated for use in Western Blot, and can be used to assess mitochondrial membrane integrity during and after purification.

Contains:

50 µg of Porin clone #2B12AF12 - Human VDAC1. Concentration 2 µg/mL.

50 µg of Cyclophilin D clone #E11AE12BD4 - Rat CypD/PPIF. Concentration 2 µg/mL.

50 µg of C-III-Core 1 clone # 16D10AD9AH5 - Bovine Heart Complex II/III. Concentration 2 µg/mL.

5 µg of Cytochrome C clone #37BA11 - Human Cytochrome c. Concentration 0.1 µg/mL.

100 µg of C-V-a clone #7H10BD4F9 - Bovine Complex V. Concentration 4 µg/mL.

This antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of 10.2 µg/mL for use in Western blot applications.

Target Information

Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC contitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 16D10AD9AH5; 20B12AF2; 37BA11; 7H10BD4F9; BR1-VDAC; Brain-derived voltage-dependent anion channel 1; Complex III subunit 4; Complex III subunit IV; CYC1; cyclophilin 3; Cyclophilin D; Cyclophilin F; CYCS; CyP-D; CyP-M; Cytochrome b-c1 complex subunit 4; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-1; cytochrome c1; Cytochrome c1, heme protein, mitochondrial; E11AE12BD4; Mitochondrial cyclophilin; mitochondrial Cyclophilin D; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; peptidylprolyl isomerase F; Plasmalemmal porin; Porin 31HL; Porin 31HM; PPIase F; Rotamase F; rVDAC1; sperm binding protein 1a; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; unnamed protein product; VDAC-1; VDAC-5; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5

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Gene Aliases: 2610002H19Rik; AA408921; AL033343; AW457192; CYC; CYC1; CYCS; CYCSA; CyP-D; CyP-F; CyP-M; CYP3; CypD; cytc; HCS; MC3DN6; PORIN; PPIase; PPIF; THC4; UQCR4; VDAC; VDAC-1; VDAC1; Vdac5

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UniProt ID: (Bovine) P00125, (Human) P08574, (Human) P99999, (Bovine) P62894, (Mouse) Q9D0M3, (Mouse) P62897, (Rat) P62898, (Human) P30405, (Bovine) P30404, (Rat) P29117, (Mouse) Q99KR7, (Bovine) P45879, (Human) P21796, (Mouse) Q60932, (Rat) Q9Z2L0

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Entrez Gene ID: (Bovine) 512500, (Human) 1537, (Human) 54205, (Bovine) 510767, (Rat) 300047, (Mouse) 66445, (Mouse) 13063, (Rat) 25309, (Human) 10105, (Bovine) 414346, (Rat) 282819, (Mouse) 105675, (Bovine) 282119, (Human) 7416, (Mouse) 22333, (Rat) 83529

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Function(s)
heme binding metal ion binding protein binding ubiquinol-cytochrome-c reductase activity electron carrier activity molecular_function iron ion binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity lipid binding peptidyl-prolyl cis-trans isomerase activity cyclosporin A binding isomerase activity peptide binding nucleotide binding voltage-gated anion channel activity porin activity voltage-gated ion channel activity ATP binding oxysterol binding cholesterol binding protein kinase binding phosphatidylcholine binding identical protein binding ion channel binding ceramide binding anion channel activity protein complex binding voltage-gated ion channel chaperone
Process(es)
mitochondrial electron transport, ubiquinol to cytochrome c response to glucagon cellular respiration hydrogen ion transmembrane transport mitochondrial electron transport, cytochrome c to oxygen apoptotic process apoptotic signaling pathway intrinsic apoptotic signaling pathway execution phase of apoptosis transport oxidation-reduction process apoptotic DNA fragmentation activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration protein folding mitochondrion organization apoptotic mitochondrial changes regulation of proton-transporting ATPase activity, rotational mechanism programmed cell death negative regulation of apoptotic process regulation of mitochondrial membrane permeability mitochondrial depolarization muscle structure development necroptotic process cellular response to hydrogen peroxide cellular response to arsenic-containing substance cellular response to calcium ion negative regulation of release of cytochrome c from mitochondria negative regulation of oxidative phosphorylation skeletal muscle fiber differentiation regulation of mitochondrial membrane permeability involved in programmed necrotic cell death mitochondrial outer membrane permeabilization involved in programmed cell death negative regulation of intrinsic apoptotic signaling pathway protein peptidyl-prolyl isomerization negative regulation of ATPase activity positive regulation of release of cytochrome c from mitochondria negative regulation of oxidative phosphorylation uncoupler activity regulation of necrotic cell death regulation of apoptotic process anion transport inorganic anion transport anion transmembrane transport behavioral fear response ion transport lipid transport chemical synaptic transmission neuron-neuron synaptic transmission learning epithelial cell differentiation calcium ion transmembrane import into mitochondrion positive regulation of apoptotic process transmembrane transport negative regulation of calcium import into the mitochondrion regulation of mitophagy regulation of mitophagy positive regulation of type 2 mitophagy mitochondrial transmembrane transport negative regulation of reactive oxygen species metabolic process mitochondrial calcium ion transport regulation of anion transport regulation of anion transmembrane transport ion transmembrane transport regulation of ion transmembrane transport
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