Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 546 conjugate was performed using HeLa cells stained with alpha Tubulin (23610501) Mouse Monoclonal Primary Antibody (A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2ug/ml mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 546 conjugate (A11030) was used at a concentration of 4ug/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 546|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:1000|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
To minimize cross-reactivity, these goat anti-mouse IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.
Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.
Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection.
A-11030 was used in immunohistochemistry - frozen section to study the neuroprotective effect of latanoprost
|Yamamoto K,Sato K,Yukita M,Yasuda M,Omodaka K,Ryu M,Fujita K,Nishiguchi KM,Machida S,Nakazawa T||Journal of neurochemistry (140:495)||2017|
CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis.
A-11030 was used in immunohistochemistry - frozen section to determine how induced arthritis can be effectively treated by CTRP6
|Murayama MA,Kakuta S,Inoue A,Umeda N,Yonezawa T,Maruhashi T,Tateishi K,Ishigame H,Yabe R,Ikeda S,Seno A,Chi HH,Hashiguchi Y,Kurata R,Tada T,Kubo S,Sato N,Liu Y,Hattori M,Saijo S,Matsushita M,Fujita T,Sumida T,Iwakura Y||Nature communications (6:null)||2015|
Three-dimensional podocyte-endothelial cell co-cultures: Assembly, validation, and application to drug testing and intercellular signaling studies.
A-11030 was used in immunocytochemistry to report a protocol to obtain and utilize a three-dimensional podocyte-endothelial co-culture to measure albumin permeability
|Li M,Corbelli A,Watanabe S,Armelloni S,Ikehata M,Parazzi V,Pignatari C,Giardino L,Mattinzoli D,Lazzari L,Puliti A,Cellesi F,Zennaro C,Messa P,Rastaldi MP||European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (86:1)||2016|
Accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4 in aggresomes.
A-11030 was used in immunocytochemistry to assess aggresomes with an accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4
|Ádám C,Fekete A,B¿gel G,Németh Z,T¿kési N,Ovádi J,Liliom K,Pesti S,Geiszt M,Buday L||Cell communication and signaling : CCS (13:null)||2015|
|Not Applicable||Not Cited||
Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism.
A-11030 was used in immunocytochemistry and immunohistochemistry - paraffin section to elucidate how optineurin contributes to disease
|Shen WC,Li HY,Chen GC,Chern Y,Tu PH||Autophagy (11:685)||2015|
|Not Applicable||Not Cited||
The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis.
A-11030 was used in immunocytochemistry to identify PACRG as a gene that contributes to flagella regulation
|Dawe HR,Farr H,Portman N,Shaw MK,Gull K||Journal of cell science (118:5421)||2005|
Adult-born and preexisting olfactory granule neurons undergo distinct experience-dependent modifications of their olfactory responses in vivo.
A-11030 was used in immunocytochemistry to test if recently generated, adult-born neurons contribute to neural plasticity and learning
|Magavi SS,Mitchell BD,Szentirmai O,Carter BS,Macklis JD||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:10729)||2005|
GAP-43 mRNA in growth cones is associated with HuD and ribosomes.
A-11030 was used in immunocytochemistry study mechanisms of GAP-43 mRNA translation and stabilization in growth cones
|Smith CL,Afroz R,Bassell GJ,Furneaux HM,Perrone-Bizzozero NI,Burry RW||Journal of neurobiology (61:222)||2004|
|Not Applicable||Not Cited||Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.||Pitulescu ME,Schmidt I,Benedito R,Adams RH||Nature protocols (5:1518)||2010|
|Not Applicable||Not Cited||Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor.||Hirota N,Yasuda D,Hashidate T,Yamamoto T,Yamaguchi S,Nagamune T,Nagase T,Shimizu T,Nakamura M||The Journal of biological chemistry (285:5931)||2010|
|Not Applicable||Not Cited||Insoluble, speckled cytosolic distribution of retinoic acid receptor alpha protein as a marker of hepatic stellate cell activation in vitro.||Mezaki Y,Yamaguchi N,Yoshikawa K,Miura M,Imai K,Itoh H,Senoo H||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (57:687)||2009|
|Not Applicable||Not Cited||Design of the artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells.||Nagaoka M,Hagiwara Y,Takemura K,Murakami Y,Li J,Duncan SA,Akaike T||The Journal of biological chemistry (283:26468)||2008|
|Not Applicable||Not Cited||Extensive fusion of haematopoietic cells with Purkinje neurons in response to chronic inflammation.||Johansson CB,Youssef S,Koleckar K,Holbrook C,Doyonnas R,Corbel SY,Steinman L,Rossi FM,Blau HM||Nature cell biology (10:575)||2008|
|Not Applicable||Not Cited||Caveolin regulates endocytosis of the muscle repair protein, dysferlin.||Hernández-Deviez DJ,Howes MT,Laval SH,Bushby K,Hancock JF,Parton RG||The Journal of biological chemistry (283:6476)||2008|
|Not Applicable||Not Cited||Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.||Sakaue-Sawano A,Kurokawa H,Morimura T,Hanyu A,Hama H,Osawa H,Kashiwagi S,Fukami K,Miyata T,Miyoshi H,Imamura T,Ogawa M,Masai H,Miyawaki A||Cell (132:487)||2008|
|Not Applicable||Not Cited||Invasive Escherichia coli are a feature of Crohn's disease.||Sasaki M,Sitaraman SV,Babbin BA,Gerner-Smidt P,Ribot EM,Garrett N,Alpern JA,Akyildiz A,Theiss AL,Nusrat A,Klapproth JM||Laboratory investigation; a journal of technical methods and pathology (87:1042)||2007|
|Not Applicable||Not Cited||Distinct roles of TIR and non-TIR regions in the subcellular localization and signaling properties of MyD88.||Nishiya T,Kajita E,Horinouchi T,Nishimoto A,Miwa S||FEBS letters (581:3223)||2007|
|Not Applicable||Not Cited||Phase-dependent roles of reactive microglia and astrocytes in nervous system injury as delineated by imaging of peripheral benzodiazepine receptor.||Maeda J,Higuchi M,Inaji M,Ji B,Haneda E,Okauchi T,Zhang MR,Suzuki K,Suhara T||Brain research (1157:100)||2007|
|Not Applicable||Not Cited||Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity.||Ghrebi SS,Owen GR,Brunette DM||Microscopy research and technique (70:555)||2007|
|Not Applicable||Not Cited||Activity-dependent validation of excitatory versus inhibitory synapses by neuroligin-1 versus neuroligin-2.||Chubykin AA,Atasoy D,Etherton MR,Brose N,Kavalali ET,Gibson JR,Südhof TC||Neuron (54:919)||2007|
|Not Applicable||Not Cited||Direct visualization of glucocorticoid receptor positive cells in the hippocampal regions using green fluorescent protein transgenic mice.||Nishi M,Usuku T,Itose M,Fujikawa K,Hosokawa K,Matsuda KI,Kawata M||Neuroscience (146:1555)||2007|
|Not Applicable||Not Cited||Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.||Weng N,Thomas DD,Groblewski GE||The Journal of biological chemistry (282:9635)||2007|
|Not Applicable||Not Cited||Stimulation of endogenous neurogenesis by anti-EFRH immunization in a transgenic mouse model of Alzheimer's disease.||Becker M,Lavie V,Solomon B||Proceedings of the National Academy of Sciences of the United States of America (104:1691)||2007|
|Not Applicable||Not Cited||Internalization of novel non-viral vector TAT-streptavidin into human cells.||Rinne J,Albarran B,Jylhävä J,Ihalainen TO,Kankaanpää P,Hytönen VP,Stayton PS,Kulomaa MS,Vihinen-Ranta M||BMC biotechnology (7:null)||2007|
|Not Applicable||Not Cited||2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy.||Staudt T,Lang MC,Medda R,Engelhardt J,Hell SW||Microscopy research and technique (70:1)||2007|
|Not Applicable||Not Cited||Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle progression.||Gangwani L||The Journal of biological chemistry (281:40330)||2006|
|Not Applicable||Not Cited||HuD distribution changes in response to heat shock but not neurotrophic stimulation.||Burry RW,Smith CL||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (54:1129)||2006|
|Not Applicable||Not Cited||Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production.||Nakanishi H,Shindou H,Hishikawa D,Harayama T,Ogasawara R,Suwabe A,Taguchi R,Shimizu T||The Journal of biological chemistry (281:20140)||2006|
|Not Applicable||Not Cited||A new motif necessary and sufficient for stable localization of the delta2 glutamate receptors at postsynaptic spines.||Matsuda S,Matsuda K,Yuzaki M||The Journal of biological chemistry (281:17501)||2006|
|Not Applicable||Not Cited||Systematic characterization of nuclear proteome during apoptosis: a quantitative proteomic study by differential extraction and stable isotope labeling.||Hwang SI,Lundgren DH,Mayya V,Rezaul K,Cowan AE,Eng JK,Han DK||Molecular and cellular proteomics : MCP (5:1131)||2006|
|Not Applicable||Not Cited||Direct membrane protein-DNA interactions required early in nuclear envelope assembly.||Ulbert S,Platani M,Boue S,Mattaj IW||The Journal of cell biology (173:469)||2006|
|Not Applicable||Not Cited||The adaptor protein Nck interacts with Fas ligand: Guiding the death factor to the cytotoxic immunological synapse.||Lettau M,Qian J,Linkermann A,Latreille M,Larose L,Kabelitz D,Janssen O||Proceedings of the National Academy of Sciences of the United States of America (103:5911)||2006|
|Not Applicable||Not Cited||Prion infection of mouse neurospheres.||Giri RK,Young R,Pitstick R,DeArmond SJ,Prusiner SB,Carlson GA||Proceedings of the National Academy of Sciences of the United States of America (103:3875)||2006|
|Not Applicable||Not Cited||Lamin A/C expression is a marker of mouse and human embryonic stem cell differentiation.||Constantinescu D,Gray HL,Sammak PJ,Schatten GP,Csoka AB||Stem cells (Dayton, Ohio) (24:177)||2006|
|Not Applicable||Not Cited||Initiation of embryonic cardiac pacemaker activity by inositol 1,4,5-trisphosphate-dependent calcium signaling.||Méry A,Aimond F,Ménard C,Mikoshiba K,Michalak M,Pucéat M||Molecular biology of the cell (16:2414)||2005|
|Not Applicable||Not Cited||Improvement in nuclear entry and transgene expression of baculoviruses by disintegration of microtubules in human hepatocytes.||Salminen M,Airenne KJ,Rinnankoski R,Reimari J,Välilehto O,Rinne J,Suikkanen S,Kukkonen S,Ylä-Herttuala S,Kulomaa MS,Vihinen-Ranta M||Journal of virology (79:2720)||2005|
|Not Applicable||Not Cited||Vaccinia virus F1L protein is a tail-anchored protein that functions at the mitochondria to inhibit apoptosis.||Stewart TL,Wasilenko ST,Barry M||Journal of virology (79:1084)||2005|
|Not Applicable||Not Cited||Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110.||Gatesman A,Walker VG,Baisden JM,Weed SA,Flynn DC||Molecular and cellular biology (24:7578)||2004|
|Not Applicable||Not Cited||Characterization of gabaergic neurons within the human medial mamillary nucleus.||Dixon G,Garrick T,Whiteman I,Sarris M,Sithamparanathan S,Harper CG||Neuroscience (127:365)||2004|
|Not Applicable||Not Cited||A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii.||Carey KL,Westwood NJ,Mitchison TJ,Ward GE||Proceedings of the National Academy of Sciences of the United States of America (101:7433)||2004|
|Not Applicable||Not Cited||The tyrp1-Tag/tyrp1-FGFR1-DN bigenic mouse: a model for selective inhibition of tumor development, angiogenesis, and invasion into the neural tissue by blockade of fibroblast growth factor receptor activity.||Rousseau B,Larrieu-Lahargue F,Javerzat S,Guilhem-Ducléon F,Beermann F,Bikfalvi A||Cancer research (64:2490)||2004|
|Not Applicable||Not Cited||Regulation of mechanical interactions between fibroblasts and the substratum by stretch-activated Ca2+ entry.||Munevar S,Wang YL,Dembo M||Journal of cell science (117:85)||2004|
|Not Applicable||Not Cited||Vaccinia virus encodes a previously uncharacterized mitochondrial-associated inhibitor of apoptosis.||Wasilenko ST,Stewart TL,Meyers AF,Barry M||Proceedings of the National Academy of Sciences of the United States of America (100:14345)||2003|
|Not Applicable||Not Cited||HuC/D confocal imaging points to olfactory migratory cells as the first cell population that expresses a post-mitotic neuronal phenotype in the chick embryo.||Fornaro M,Geuna S,Fasolo A,Giacobini-Robecchi MG||Neuroscience (122:123)||2003|
|Not Applicable||Not Cited||Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release.||Wucherpfennig T,Wilsch-Bräuninger M,González-Gaitán M||The Journal of cell biology (161:609)||2003|
|Not Applicable||Not Cited||Casein kinase I epsilon enhances the binding of Dvl-1 to Frat-1 and is essential for Wnt-3a-induced accumulation of beta-catenin.||Hino S,Michiue T,Asashima M,Kikuchi A||The Journal of biological chemistry (278:14066)||2003|
|Not Applicable||Not Cited||Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.||Hering H,Lin CC,Sheng M||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:3262)||2003|
|Not Applicable||Not Cited||22R-hydroxycholesterol and 9-cis-retinoic acid induce ATP-binding cassette transporter A1 expression and cholesterol efflux in brain cells and decrease amyloid beta secretion.||Koldamova RP,Lefterov IM,Ikonomovic MD,Skoko J,Lefterov PI,Isanski BA,DeKosky ST,Lazo JS||The Journal of biological chemistry (278:13244)||2003|
|Not Applicable||Not Cited||Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.||Premzl A,Zavasnik-Bergant V,Turk V,Kos J||Experimental cell research (283:206)||2003|
|Not Applicable||Not Cited||Cloning and characterization of four Anopheles gambiae serpin isoforms, differentially induced in the midgut by Plasmodium berghei invasion.||Danielli A,Kafatos FC,Loukeris TG||The Journal of biological chemistry (278:4184)||2003|
|Not Applicable||Not Cited||Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway.||Goshima G,Kiyomitsu T,Yoda K,Yanagida M||The Journal of cell biology (160:25)||2003|
|Not Applicable||Not Cited||Validation and quantitation of an in vitro M-cell model.||Tyrer P,Ruth Foxwell A,Kyd J,Harvey M,Sizer P,Cripps A||Biochemical and biophysical research communications (299:377)||2002|
|Not Applicable||Not Cited||Aggregation of f-actin in olfactory glomeruli: a common feature of glomeruli across phyla.||Rössler W,Kuduz J,Schürmann FW,Schild D||Chemical senses (27:803)||2002|
|Not Applicable||Not Cited||MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1.||Lim R,Winteringham LN,Williams JH,McCulloch RK,Ingley E,Tiao JY,Lalonde JP,Tsai S,Tilbrook PA,Sun Y,Wu X,Morris SW,Klinken SP||The Journal of biological chemistry (277:40997)||2002|
|Not Applicable||Not Cited||Chemokine stimulation of lymphocyte alpha 4 integrin avidity but not of leukocyte function-associated antigen-1 avidity to endothelial ligands under shear flow requires cholesterol membrane rafts.||Shamri R,Grabovsky V,Feigelson SW,Dwir O,Van Kooyk Y,Alon R||The Journal of biological chemistry (277:40027)||2002|
|Not Applicable||Not Cited||Platelets, circulating tissue factor, and fibrin colocalize in ex vivo thrombi: real-time fluorescence images of thrombus formation and propagation under defined flow conditions.||Balasubramanian V,Grabowski E,Bini A,Nemerson Y||Blood (100:2787)||2002|
|Not Applicable||Not Cited||A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin.||Rizzo MA,Magnuson MA,Drain PF,Piston DW||The Journal of biological chemistry (277:34168)||2002|
|Not Applicable||Not Cited||Roles of fission yeast tea1p in the localization of polarity factors and in organizing the microtubular cytoskeleton.||Behrens R,Nurse P||The Journal of cell biology (157:783)||2002|
|Not Applicable||Not Cited||Pincher, a pinocytic chaperone for nerve growth factor/TrkA signaling endosomes.||Shao Y,Akmentin W,Toledo-Aral JJ,Rosenbaum J,Valdez G,Cabot JB,Hilbush BS,Halegoua S||The Journal of cell biology (157:679)||2002|
|Not Applicable||Not Cited||Characterization of a mammalian Golgi-localized protein complex, COG, that is required for normal Golgi morphology and function.||Ungar D,Oka T,Brittle EE,Vasile E,Lupashin VV,Chatterton JE,Heuser JE,Krieger M,Waters MG||The Journal of cell biology (157:405)||2002|
|Not Applicable||Not Cited||SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila.||Zallen JA,Cohen Y,Hudson AM,Cooley L,Wieschaus E,Schejter ED||The Journal of cell biology (156:689)||2002|
|Not Applicable||Not Cited||Transforming growth factor-beta 1 regulates Kir2.3 inward rectifier K+ channels via phospholipase C and protein kinase C-delta in reactive astrocytes from adult rat brain.||Perillan PR,Chen M,Potts EA,Simard JM||The Journal of biological chemistry (277:1974)||2002|
|Not Applicable||Not Cited||Immunofluorescence technique for 100-nm-thick semithin sections of Epon-embedded tissues.||Haraguchi CM,Yokota S||Histochemistry and cell biology (117:81)||2002|
|Not Applicable||Not Cited||Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae.||Mironov AA,Beznoussenko GV,Nicoziani P,Martella O,Trucco A,Kweon HS,Di Giandomenico D,Polishchuk RS,Fusella A,Lupetti P,Berger EG,Geerts WJ,Koster AJ,Burger KN,Luini A||The Journal of cell biology (155:1225)||2001|
|Not Applicable||Not Cited||Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone.||Rape M,Hoppe T,Gorr I,Kalocay M,Richly H,Jentsch S||Cell (107:667)||2001|
|Not Applicable||Not Cited||Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins.||Annaert WG,Esselens C,Baert V,Boeve C,Snellings G,Cupers P,Craessaerts K,De Strooper B||Neuron (32:579)||2001|
|Not Applicable||Not Cited||Distinct retrieval and retention mechanisms are required for the quality control of endoplasmic reticulum protein folding.||Vashist S,Kim W,Belden WJ,Spear ED,Barlowe C,Ng DT||The Journal of cell biology (155:355)||2001|
|Not Applicable||Not Cited||Interaction between pyrin and the apoptotic speck protein (ASC) modulates ASC-induced apoptosis.||Richards N,Schaner P,Diaz A,Stuckey J,Shelden E,Wadhwa A,Gumucio DL||The Journal of biological chemistry (276:39320)||2001|
|Not Applicable||Not Cited||The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis.||Raasi S,Schmidtke G,Groettrup M||The Journal of biological chemistry (276:35334)||2001|
|Not Applicable||Not Cited||Combinatorial roles of olig2 and neurogenin2 in the coordinated induction of pan-neuronal and subtype-specific properties of motoneurons.||Mizuguchi R,Sugimori M,Takebayashi H,Kosako H,Nagao M,Yoshida S,Nabeshima Y,Shimamura K,Nakafuku M||Neuron (31:757)||2001|
|Not Applicable||Not Cited||U box proteins as a new family of ubiquitin-protein ligases.||Hatakeyama S,Yada M,Matsumoto M,Ishida N,Nakayama KI||The Journal of biological chemistry (276:33111)||2001|
|Not Applicable||Not Cited||The discrepancy between presenilin subcellular localization and gamma-secretase processing of amyloid precursor protein.||Cupers P,Bentahir M,Craessaerts K,Orlans I,Vanderstichele H,Saftig P,De Strooper B,Annaert W||The Journal of cell biology (154:731)||2001|
|Not Applicable||Not Cited||Catalytic subunit of protein kinase A is an interacting partner of the inflammation-responsive transcription factor serum amyloid A-activating factor-1.||Ray BK,Chen J,Ray A||Journal of immunology (Baltimore, Md. : 1950) (167:2343)||2001|
|Not Applicable||Not Cited||The chaperone protein 14-3-3eta interacts with the nicotinic acetylcholine receptor alpha 4 subunit. Evidence for a dynamic role in subunit stabilization.||Jeanclos EM,Lin L,Treuil MW,Rao J,DeCoster MA,Anand R||The Journal of biological chemistry (276:28281)||2001|
|Not Applicable||Not Cited||Localization of yeast telomeres to the nuclear periphery is separable from transcriptional repression and telomere stability functions.||Tham WH,Wyithe JS,Ko Ferrigno P,Silver PA,Zakian VA||Molecular cell (8:189)||2001|
|Not Applicable||Not Cited||An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin.||Imai Y,Soda M,Inoue H,Hattori N,Mizuno Y,Takahashi R||Cell (105:891)||2001|
|Not Applicable||Not Cited||Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides.||Martel V,Racaud-Sultan C,Dupe S,Marie C,Paulhe F,Galmiche A,Block MR,Albiges-Rizo C||The Journal of biological chemistry (276:21217)||2001|
|Not Applicable||Not Cited||A mouse homologue of the Drosophila tumor suppressor l(2)tid gene defines a novel Ras GTPase-activating protein (RasGAP)-binding protein.||Trentin GA,Yin X,Tahir S,Lhotak S,Farhang-Fallah J,Li Y,Rozakis-Adcock M||The Journal of biological chemistry (276:13087)||2001|
|Not Applicable||Not Cited||Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons.||Smith GA,Gross SP,Enquist LW||Proceedings of the National Academy of Sciences of the United States of America (98:3466)||2001|
|Not Applicable||Not Cited||Blockade of the extracellular signal-regulated kinase pathway induces marked G1 cell cycle arrest and apoptosis in tumor cells in which the pathway is constitutively activated: up-regulation of p27(Kip1).||Hoshino R,Tanimura S,Watanabe K,Kataoka T,Kohno M||The Journal of biological chemistry (276:2686)||2001|
|Not Applicable||Not Cited||p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).||King CC,Gardiner EM,Zenke FT,Bohl BP,Newton AC,Hemmings BA,Bokoch GM||The Journal of biological chemistry (275:41201)||2000|
|Not Applicable||Not Cited||Synaptic localization and presynaptic function of calcium channel beta 4-subunits in cultured hippocampal neurons.||Wittemann S,Mark MD,Rettig J,Herlitze S||The Journal of biological chemistry (275:37807)||2000|
|Not Applicable||Not Cited||Substrate flexibility regulates growth and apoptosis of normal but not transformed cells.||Wang HB,Dembo M,Wang YL||American journal of physiology. Cell physiology (279:C1345)||2000|
|Not Applicable||Not Cited||Stimulation of fascin spikes by thrombospondin-1 is mediated by the GTPases Rac and Cdc42.||Adams JC,Schwartz MA||The Journal of cell biology (150:807)||2000|
|Not Applicable||Not Cited||Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus.||Khoo KM,Han MK,Park JB,Chae SW,Kim UH,Lee HC,Bay BH,Chang CF||The Journal of biological chemistry (275:24807)||2000|
|Not Applicable||Not Cited||Regulated expression of nullo is required for the formation of distinct apical and basal adherens junctions in the Drosophila blastoderm.||Hunter C,Wieschaus E||The Journal of cell biology (150:391)||2000|
|Not Applicable||Not Cited||Mammalian spindle orientation and position respond to changes in cell shape in a dynein-dependent fashion.||O'Connell CB,Wang YL||Molecular biology of the cell (11:1765)||2000|
|Not Applicable||Not Cited||Regulation of microfilament reorganization and invasiveness of breast cancer cells by kinase dead p21-activated kinase-1.||Adam L,Vadlamudi R,Mandal M,Chernoff J,Kumar R||The Journal of biological chemistry (275:12041)||2000|
|Not Applicable||Not Cited||MAPK mediates RAS-induced chromosome instability.||Saavedra HI,Fukasawa K,Conn CW,Stambrook PJ||The Journal of biological chemistry (274:38083)||1999|
|Not Applicable||Not Cited||Novel functions of nanos in downregulating mitosis and transcription during the development of the Drosophila germline.||Deshpande G,Calhoun G,Yanowitz JL,Schedl PD||Cell (99:271)||1999|
|Not Applicable||Not Cited||Store-operated Ca2+ entry: evidence for a secretion-like coupling model.||Patterson RL,van Rossum DB,Gill DL||Cell (98:487)||1999|