Microtubules of NIH 3T3 cells labeled with mouse anti–a-tubulin monoclonal IgG<sub>1</sub> antibody (Cat. no. A11126) and visualized with blue-fluorescent Alexa Fluor® 405 goat anti–mouse IgG antibody (Cat. no. A31553). Nuclei were stained with red-fluorescent propidium iodide (Cat. no. P1304MP, P3566, P21493).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 405|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and serum prior to conjucation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration.
A-31553 was used in immunohistochemistry to characterize mice with a forebrain-specific loss of O-GlcNAc glycosylation
|Wang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC||Proceedings of the National Academy of Sciences of the United States of America (113:15120)||2016|
High-performance probes for light and electron microscopy.
A-31553 was used in immunohistochemistry to develop and characterize 'spaghetti monster' fluorescent proteins
|Viswanathan S,Williams ME,Bloss EB,Stasevich TJ,Speer CM,Nern A,Pfeiffer BD,Hooks BM,Li WP,English BP,Tian T,Henry GL,Macklin JJ,Patel R,Gerfen CR,Zhuang X,Wang Y,Rubin GM,Looger LL||Nature methods (12:568)||2015|
Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo.
A-31553 was used in immunohistochemistry to report that selective photostimulation of astrocytes with channelrhodopsin-2 in primary visual cortex enhances both excitatory and inhibitory synaptic transmission
|Perea G,Yang A,Boyden ES,Sur M||Nature communications (5:null)||2014|
The Glutamine-Alanine Repeat Domain of TCERG1 is Required for the Inhibition of the Growth Arrest Activity of C/EBP¿.
A-31553 was used in immunocytochemistry to research the inhibition of the growth arrest activity of C/EBPalpha requiring the glutamin-alanine repeat domain of TCERG1
|Miller NJ,Schick K,Timchenko N,Harrison E,Roesler WJ||Journal of cellular biochemistry (117:612)||2016|
|Not Applicable||Not Cited||Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling.||Orba Y,Suzuki T,Makino Y,Kubota K,Tanaka S,Kimura T,Sawa H||The Journal of biological chemistry (285:1544)||2010|
|Not Applicable||Not Cited||Mechanism for amyloid precursor-like protein 2 enhancement of major histocompatibility complex class I molecule degradation.||Tuli A,Sharma M,Capek HL,Naslavsky N,Caplan S,Solheim JC||The Journal of biological chemistry (284:34296)||2009|
|Not Applicable||Not Cited||Jagged-mediated Notch signaling maintains proliferating neural progenitors and regulates cell diversity in the ventral spinal cord.||Yeo SY,Chitnis AB||Proceedings of the National Academy of Sciences of the United States of America (104:5913)||2007|
|Not Applicable||Not Cited||Essential role of PDK1 in regulating endothelial cell migration.||Primo L,di Blasio L,Roca C,Droetto S,Piva R,Schaffhausen B,Bussolino F||The Journal of cell biology (176:1035)||2007|
|Not Applicable||Not Cited||The role of Pax-6 in lens regeneration.||Madhavan M,Haynes TL,Frisch NC,Call MK,Minich CM,Tsonis PA,Del Rio-Tsonis K||Proceedings of the National Academy of Sciences of the United States of America (103:14848)||2006|
|Not Applicable||Not Cited||Optimal processing method to obtain four-color confocal fluorescent images of the cytoskeleton and nucleus in three-dimensional chondrocyte cultures.||Blanc A,Tran-Khanh N,Filion D,Buschmann MD||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:1171)||2005|