|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against solid phase human serum protein, mouse IGA and IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:20,000|
|Western Blot (WB)||1:500-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitue with 2 ml distilled water.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||20 µg/ml||
Generation and characterization of Smac/DIABLO-deficient mice.
61-6800 was used in blocking assay to characterize Smac(-/-) mice.
|Okada H,Suh WK,Jin J,Woo M,Du C,Elia A,Duncan GS,Wakeham A,Itie A,Lowe SW,Wang X,Mak TW||Molecular and cellular biology (22:3509)||2002|
|Not Applicable||Not Cited||
The stress kinase mitogen-activated protein kinase kinase (MKK)7 is a negative regulator of antigen receptor and growth factor receptor-induced proliferation in hematopoietic cells.
61-6800 was used in blocking assay to assess the physiological role of MKK7 in hematopoietic cells.
|Sasaki T,Wada T,Kishimoto H,Irie-Sasaki J,Matsumoto G,Goto T,Yao Z,Wakeham A,Mak TW,Suzuki A,Cho SK,Zuniga-Pflucker JC,Oliveira-dos-Santos AJ,Katada T,Nishina H,Penninger JM||The Journal of experimental medicine (194:757)||2001|
|Not Applicable||Not Cited||
Negative regulation of lymphocyte activation and autoimmunity by the molecular adaptor Cbl-b.
61-6800 was used in ELISA to use transgenic mice lacking the adaptor Cbl-b to study spontaneous autoimmunity.
|Bachmaier K,Krawczyk C,Kozieradzki I,Kong YY,Sasaki T,Oliveira-dos-Santos A,Mariathasan S,Bouchard D,Wakeham A,Itie A,Le J,Ohashi PS,Sarosi I,Nishina H,Lipkowitz S,Penninger JM||Nature (403:211)||2000|
|Not Applicable||Not Cited||A simple technique for the rapid enrichment of class and subclass hybridoma switch variants. A 1000-fold enrichment in half the time, for half the cost.||Faguet GB,Agee JF||Journal of immunological methods (165:217)||1993|
|Not Applicable||Not Cited||The identification of monoclonal class switch variants by sib selection and an ELISA assay.||Spira G,Bargellesi A,Teillaud JL,Scharff MD||Journal of immunological methods (74:307)||1984|
|Not Applicable||Not Cited||The influence of murine macrophage-conditioned medium on cloning efficiency, antibody synthesis, and growth rate of hybridomas.||Sugasawara RJ,Cahoon BE,Karu AE||Journal of immunological methods (79:263)||1985|