Invitrogen

MyD88 Polyclonal Antibody

Product Image Gallery

MyD88 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human MyD88 in human kidney with MyD88 Polyclonal Antibody (Product # PA5-118095, 1:200 dilution, formalin-fixed paraffin embedded sections).

MyD88 Antibody in Western Blot (WB) — Western Blot using MyD88 Polyclonal Antibody (Product # PA5-118095) at 1:500 dilution. Lane A: K562 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 35 kDa.

MyD88 Antibody in Immunoprecipitation (IP) — MyD88 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with MyD88 Polyclonal Antibody (Product # PA5-118095) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MyD88 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 34 kDa.

Product Details

PA5-118095

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:500	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human MYD88 fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); Myeloid differentiation primary response protein MyD88

Gene Aliases: MYD88; MYD88D

UniProt ID: (Human) Q99836

Entrez Gene ID: (Human) 4615

Molecular Function: scaffold/adaptor protein