Invitrogen

Myogenin Polyclonal Antibody

View all (12) Myogenin antibodies

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Myogenin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Myogenin in paraffin-embedded human lung cancer tissue. Samples were incubated in polyclonal Myogenin antibody (Product # PA5-110007) using a dilution of 1:100.

Myogenin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Myogenin in paraffin-embedded rat ovary tissue. Samples were incubated in polyclonal Myogenin antibody (Product # PA5-110007) using a dilution of 1:100.

Myogenin Antibody in Western Blot (WB) — Western blot analysis of Myogenin in mouse skeletal muscle, rat heart. Samples were incubated in polyclonal Myogenin antibody (Product # PA5-110007) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:200

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Western Blot (WB)

1:500-1:2000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human Myogenin (NP_002470.2).

Conjugate

Unconjugated

Form

Liquid

Concentration

0.82 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2855418

Product Specific Information

Sequence of this protein is as follows: MELYETSPYF YQEPRFYDGE NYLPVHLQGF EPPGYERTEL TLSPEAPGPL EDKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG GGGPQPGVPS ECSSHSASCS PEWGSALEFS ANPGDHLLTA DPTDAHNLHS LTSIVDSITV EDVSVAFPDE TMPN

Target Information

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYOD1-related protein; Myogenic factor 4; Myogenic factor-4; Myogenin

Gene Aliases: BHLHC3; myf-4; MYF4; myo; MYOG

UniProt ID: (Human) P15173, (Mouse) P12979, (Rat) P20428

Entrez Gene ID: (Human) 4656, (Mouse) 17928, (Rat) 29148

Molecular Function: basic helix-loop-helix transcription factor nucleic acid binding transcription factor

ossification cell cycle skeletal muscle tissue development negative regulation of cell proliferation response to gravity positive regulation of myotube differentiation skeletal muscle atrophy positive regulation of muscle atrophy regulation of skeletal muscle satellite cell proliferation response to muscle activity involved in regulation of muscle adaptation response to electrical stimulus involved in regulation of muscle adaptation striated muscle atrophy response to denervation involved in regulation of muscle adaptation skeletal muscle cell differentiation muscle cell fate commitment mRNA transcription from RNA polymerase II promoter skeletal muscle tissue regeneration myoblast differentiation positive regulation of myoblast differentiation negative regulation of glycolytic process positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation positive regulation of cell cycle arrest cellular response to magnetism cellular response to lithium ion cellular response to retinoic acid cellular response to growth factor stimulus cellular response to estradiol stimulus regulation of myoblast fusion positive regulation of myoblast fusion positive regulation of oxidative phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development muscle organ development myotube differentiation cell differentiation histone H3 acetylation histone H4 acetylation positive regulation of transcription, DNA-templated response to muscle activity

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity E-box binding core promoter proximal region sequence-specific DNA binding DNA binding protein dimerization activity molecular_function