Invitrogen

Myogenin Polyclonal Antibody

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Myogenin Antibody in Western Blot (WB) — Western blot analysis of Myogenin in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with Myogenin polyclonal antibody (Product # PA5-78067) using a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Myogenin. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/ml

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2736181

Product Specific Information

Positive Control: RD

Predicted Reactivity: Mouse (95%), Rat (95%), Pig (95%), Rabbit (96%), Sheep (92%), Bovine (92%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix proteins. It is essential for the development of functional skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOG; Myogenic factor 4; Myogenin; myogenin (myogenic factor 4)

Gene Aliases: BHLHC3; myf-4; MYF4; MYOG

UniProt ID: (Human) P15173

Entrez Gene ID: (Human) 4656

Molecular Function: basic helix-loop-helix transcription factor nucleic acid binding transcription factor

ossification cell cycle skeletal muscle tissue development negative regulation of cell proliferation response to gravity positive regulation of myotube differentiation skeletal muscle atrophy positive regulation of muscle atrophy regulation of skeletal muscle satellite cell proliferation response to muscle activity involved in regulation of muscle adaptation response to electrical stimulus involved in regulation of muscle adaptation striated muscle atrophy response to denervation involved in regulation of muscle adaptation skeletal muscle cell differentiation muscle cell fate commitment mRNA transcription from RNA polymerase II promoter skeletal muscle tissue regeneration myoblast differentiation positive regulation of myoblast differentiation negative regulation of glycolytic process positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation positive regulation of cell cycle arrest cellular response to magnetism cellular response to lithium ion cellular response to retinoic acid cellular response to growth factor stimulus cellular response to estradiol stimulus regulation of myoblast fusion positive regulation of myoblast fusion positive regulation of oxidative phosphorylation

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity E-box binding