Invitrogen

NALP12 Polyclonal Antibody

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View all (9) NALP12 antibodies

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NALP12 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of NALP12 in human brain tissue with NALP12 Polyclonal Antibody (Product # PA5-21027) at 5 µg/mL.

NALP12 Antibody in Immunohistochemistry (IHC) — Immunofluorescence of NALP12 in human brain tissue with NALP12 Polyclonal Antibody (Product # PA5-21027) at 20 µg/mL.

NALP12 Antibody in Western Blot (WB) — Western Blot analysis of NALP12 in human brain tissue lysate with NALP12 Polyclonal Antibody (Product # PA5-21027) at 1 µg/mL.

NALP12 Antibody in Western Blot (WB) — Figure 5. IRF3, TAB1 and NLRP12 are decreased upon infection with SARS-CoV-2 in 293T-ACE2 cells, in two independent laboratories. (A-C) 293T-ACE2 cells were infected with SARS-CoV-2 and analysed for viral and host protein levels 72 h post-infection. (D-I) Independently, stable 293T-ACE2 were infected with icSARS-CoV-2 mNeonGreen) and the levels of IRF3, TAB1 And NLRP12 were visualized by Western Blotting at 6 h (D), 24 h (E) and 48 h (F). (G-I): Protein levels measured by densitometry for IRF3 (G), TAB1 (H) and NLRP12 (I) and plotted for the Mock, poly(I:C) and SARS-CoV-2 infection at 6 h, 24 h and 48 h.

Product Details

PA5-21027

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

5 µg/mL

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide near the amino terminus of human NALP12.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11152858

Product Specific Information

A suggested positive control is human brain tissue lysate.

PA5-21027 can be used with blocking peptide PEP-1141.

Target Information

NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-kappa-B activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species.

Emerging microbes & infections

Moustaqil M,Ollivier E,Chiu HP,Van Tol S,Rudolffi-Soto P,Stevens C,Bhumkar A,Hunter DJB,Freiberg AN,Jacques D,Lee B,Sierecki E,Gambin Y

PA5-21027 was used in Western Blot to reveal that two viral proteases encoded by SARS-CoV-2 can cleave host innate immune proteins.

Wed Dec 01 00:00:00 EST 2021

SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species.

Emerging microbes & infections

Moustaqil M,Ollivier E,Chiu HP,Van Tol S,Rudolffi-Soto P,Stevens C,Bhumkar A,Hunter DJB,Freiberg AN,Jacques D,Lee B,Sierecki E,Gambin Y

PA5-21027 was used in Western Blot to reveal that two viral proteases encoded by SARS-CoV-2 can cleave host innate immune proteins.

Wed Dec 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: monarch 1; Monarch-1; NACHT, leucine rich repeat and PYD containing 12; NACHT, LRR and PYD containing protein 12; NACHT, LRR and PYD domains-containing protein 12; NLR family, pyrin domain containing 12; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12; PYPAF7; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide

Gene Aliases: CLR19.3; FCAS2; NALP12; NLRP12; PAN6; PYPAF7; RNO; RNO2

UniProt ID: (Human) P59046, (Mouse) E9Q5R7

Entrez Gene ID: (Human) 91662, (Rat) 292541, (Mouse) 378425

activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction release of cytoplasmic sequestered NF-kappaB negative regulation of signal transduction negative regulation of protein autophosphorylation negative regulation of NF-kappaB transcription factor activity dendritic cell migration regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MHC class I biosynthetic process regulation of interleukin-18 biosynthetic process negative regulation of interleukin-6 biosynthetic process negative regulation of Toll signaling pathway negative regulation of cytokine secretion negative regulation of interleukin-1 secretion positive regulation of interleukin-1 beta secretion negative regulation of inflammatory response positive regulation of inflammatory response negative regulation of ERK1 and ERK2 cascade cellular response to cytokine stimulus negative regulation of NIK/NF-kappaB signaling

Process(es)

protein binding ATP binding cysteine-type endopeptidase activator activity involved in apoptotic process nucleotide binding