Proteintech

NAMPT/PBEF Polyclonal Antibody

NAMPT/PBEF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

NAMPT/PBEF Antibody (11776-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 11776-1-AP (NAMPT/PBEF antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NAMPT/PBEF Antibody in Western Blot (WB)

NAMPT/PBEF Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11776-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NAMPT/PBEF Fusion Protein Ag2434 (1-350 aa encoded by BC020691)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNPAAEAEF NILLATDSYK VTHYKQYPPN TSKVYSYFEC REKKTENSKL RKVKYEETVF YGLQYILNKY LKGKVVTKEK IQEAKDVYKE HFQDDVFNEK GWNYILEKYD GHLPIEIKAV PEGFVIPRGN VLFTVENTDP ECYWLTNWIE TILVQSWYPI TVATNSREQK KILAKYLLET SGNLDGLEYK LHDFGYRGVS SQETAGIGAS AHLVNFKGTD TVAGLALIKK YYGTKDPVPG YSVPAAEHST ITAWGKDHEK DAFEHIVTQF SSVPVSVVSD SYDIYNACEK IWGEDLRHLI VSRSTQAPLI IRPDSGNPLD TVLKVLEILG KKFPVTENSK GYKLLPPYLR V (1-350 aa encoded by BC020691)

Target Information

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Adipokine Visfatin Modulates Cancer Stem Cell Properties in Triple-Negative Breast Cancer.

Biomedicines

Chiang YF,Huang KC,Chen HY,Huang TC,Ali M,Chang HY,Shieh TM,Shih YH,Wang KL,Huang YJ,Chung CP,Hsia SM

Fri Jan 20 00:00:00 EST 2023

Targeting NAD metabolism regulates extracellular adenosine levels to improve the cytotoxicity of CD8+ effector T cells in the tumor microenvironment of gastric cancer.

Journal of cancer research and clinical oncology

Liu HY,Wang FH,Liang JM,Xiang YY,Liu SH,Zhang SW,Zhu CM,He YL,Zhang CH

Sat Jul 01 00:00:00 EDT 2023

Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish.

Animal cells and systems

Koun S,Park HJ,Jung SM,Cha JJ,Cha DR,Kang YS

Tue Apr 25 00:00:00 EDT 2023

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a "One-Stone-Two-Birds" manner.

Journal of nanobiotechnology

Wu G,Su Q,Li J,Xue C,Zhu J,Cai Q,Huang J,Ji S,Cheng B,Ge H

Thu Jan 05 00:00:00 EST 2023

The NAMPT Inhibitor FK866 in Combination with Cisplatin Reduces Cholangiocarcinoma Cells Growth.

Cells

Pant K,Richard S,Peixoto E,Yin J,Seelig DM,Carotenuto P,Salati M,Franco B,Roberts LR,Gradilone SA

Tue Feb 28 00:00:00 EST 2023

The NAMPT Inhibitor FK866 in Combination with Cisplatin Reduces Cholangiocarcinoma Cells Growth.

Cells

Pant K,Richard S,Peixoto E,Yin J,Seelig DM,Carotenuto P,Salati M,Franco B,Roberts LR,Gradilone SA

Tue Feb 28 00:00:00 EST 2023

The NAMPT Inhibitor FK866 in Combination with Cisplatin Reduces Cholangiocarcinoma Cells Growth.

Cells

Pant K,Richard S,Peixoto E,Yin J,Seelig DM,Carotenuto P,Salati M,Franco B,Roberts LR,Gradilone SA

Tue Feb 28 00:00:00 EST 2023

High-Dosage NMN Promotes Ferroptosis to Suppress Lung Adenocarcinoma Growth through the NAM-Mediated SIRT1-AMPK-ACC Pathway.

Cancers

Zhang M,Cui J,Chen H,Wang Y,Kuai X,Sun S,Tang Q,Zong F,Chen Q,Wu J,Wu S

Sun Apr 23 00:00:00 EDT 2023

SZC-6, a small-molecule activator of SIRT3, attenuates cardiac hypertrophy in mice.

Acta pharmacologica Sinica

Li ZY,Lu GQ,Lu J,Wang PX,Zhang XL,Zou Y,Liu PQ

Wed Mar 01 00:00:00 EST 2023

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy.

Cell death & disease

Deng Y,Duan R,Ding W,Gu Q,Liu M,Zhou J,Sun J,Zhu J

Tue Dec 20 00:00:00 EST 2022

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy.

Cell death & disease

Deng Y,Duan R,Ding W,Gu Q,Liu M,Zhou J,Sun J,Zhu J

Tue Dec 20 00:00:00 EST 2022

Glucose-6-phosphate dehydrogenase exerts antistress effects independently of its enzymatic activity.

The Journal of biological chemistry

Jin X,Li X,Li L,Zhong B,Hong Y,Niu J,Li B

Thu Dec 01 00:00:00 EST 2022

Circular RNA triple functional domain promotes osteoarthritis' development by modulating the microRNA-136-5p/Nicotinamide phosphoribosyltransferase axis.

Bioengineered

Yang J,Li Q,Wang T,Lv K

Tue Mar 01 00:00:00 EST 2022

NAD⁺ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

International journal of molecular sciences

Li X,Li Y,Li F,Chen Q,Zhao Z,Liu X,Zhang N,Li H

Tue Mar 22 00:00:00 EDT 2022

NAD⁺ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

International journal of molecular sciences

Li X,Li Y,Li F,Chen Q,Zhao Z,Liu X,Zhang N,Li H

Tue Mar 22 00:00:00 EDT 2022

NAD⁺ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

International journal of molecular sciences

Li X,Li Y,Li F,Chen Q,Zhao Z,Liu X,Zhang N,Li H

Tue Mar 22 00:00:00 EDT 2022

NAD⁺ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.

International journal of molecular sciences

Li X,Li Y,Li F,Chen Q,Zhao Z,Liu X,Zhang N,Li H

Tue Mar 22 00:00:00 EDT 2022

Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass.

Drug design, development and therapy

Lu C,Yang W,Zhou J,Zhang Z,Gong Y,Hu F,Yu W,Dong X

Thu Sep 30 00:00:00 EDT 2021

Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass.

Drug design, development and therapy

Lu C,Yang W,Zhou J,Zhang Z,Gong Y,Hu F,Yu W,Dong X

Thu Sep 30 00:00:00 EDT 2021

Nicotinamide improves NAD⁺ levels to protect against acetaminophen-induced acute liver injury in mice.

Human & experimental toxicology

Xu J,Zhang L,Jiang R,Hu K,Hu D,Liao C,Jiang S,Yang Y,Huang J,Tang L,Li L

Mon Nov 01 00:00:00 EDT 2021

NAD⁺ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Cell metabolism

Lv H,Lv G,Chen C,Zong Q,Jiang G,Ye D,Cui X,He Y,Xiang W,Han Q,Tang L,Yang W,Wang H

Tue Jan 05 00:00:00 EST 2021

NAD⁺ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Cell metabolism

Lv H,Lv G,Chen C,Zong Q,Jiang G,Ye D,Cui X,He Y,Xiang W,Han Q,Tang L,Yang W,Wang H

Tue Jan 05 00:00:00 EST 2021

NAD⁺ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Cell metabolism

Lv H,Lv G,Chen C,Zong Q,Jiang G,Ye D,Cui X,He Y,Xiang W,Han Q,Tang L,Yang W,Wang H

Tue Jan 05 00:00:00 EST 2021

NAD⁺ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.

Cell metabolism

Lv H,Lv G,Chen C,Zong Q,Jiang G,Ye D,Cui X,He Y,Xiang W,Han Q,Tang L,Yang W,Wang H

Tue Jan 05 00:00:00 EST 2021

CD38 and MGluR1 as possible signaling molecules involved in epileptogenesis: A potential role for NAD⁺ homeostasis.

Brain research

Khodaverdian S,Dashtban-Moghadam E,Dabirmanesh B,Mirnajafi-Zadeh J,Taleb M,Khajeh K,Fathollahi Y

Sun Aug 15 00:00:00 EDT 2021

Hyperglycemia-reduced NAD⁺ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Metabolism: clinical and experimental

Li Y,Li J,Zhao C,Yang L,Qi X,Wang X,Zhou Q,Shi W

Fri Jan 01 00:00:00 EST 2021

Hyperglycemia-reduced NAD⁺ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Metabolism: clinical and experimental

Li Y,Li J,Zhao C,Yang L,Qi X,Wang X,Zhou Q,Shi W

Fri Jan 01 00:00:00 EST 2021

Hyperglycemia-reduced NAD⁺ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Metabolism: clinical and experimental

Li Y,Li J,Zhao C,Yang L,Qi X,Wang X,Zhou Q,Shi W

Fri Jan 01 00:00:00 EST 2021

Hyperglycemia-reduced NAD⁺ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Metabolism: clinical and experimental

Li Y,Li J,Zhao C,Yang L,Qi X,Wang X,Zhou Q,Shi W

Fri Jan 01 00:00:00 EST 2021

Impaired Nicotinamide Adenine Dinucleotide Biosynthesis in the Kidney of Chronic Kidney Disease.

Frontiers in physiology

Liu X,Luo D,Huang S,Liu S,Zhang B,Wang F,Lu J,Chen J,Li S

Tue Oct 05 00:00:00 EDT 2021

Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1.

Frontiers in pharmacology

Yang Y,Wang Y,He Z,Liu Y,Chen C,Wang Y,Wang DW,Wang H

Mon Sep 28 00:00:00 EDT 2020

Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1^G93A Mice.

International journal of biological sciences

Zhou Q,Zhu L,Qiu W,Liu Y,Yang F,Chen W,Xu R

Mon Nov 02 00:00:00 EST 2020

Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma.

Oncogenesis

Gibson AE,Yeung C,Issaq SH,Collins VJ,Gouzoulis M,Zhang Y,Ji J,Mendoza A,Heske CM

Thu Sep 10 00:00:00 EDT 2020

Corneal Denervation Causes Epithelial Apoptosis Through Inhibiting NAD+ Biosynthesis.

Investigative ophthalmology & visual science

Li Y,Ma X,Li J,Yang L,Zhao X,Qi X,Zhang X,Zhou Q,Shi W

Thu Aug 01 00:00:00 EDT 2019

NAMPT overexpression alleviates alcohol-induced hepatic steatosis in mice.

PloS one

Xiong X,Yu J,Fan R,Zhang C,Xu L,Sun X,Huang Y,Wang Q,Ruan HB,Qian X

Mon Nov 18 00:00:00 EST 2019

NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer.

Frontiers in oncology

Li XQ,Lei J,Mao LH,Wang QL,Xu F,Ran T,Zhou ZH,He S

Tue Sep 29 00:00:00 EDT 2020

NAMPT and NAPRT, Key Enzymes in NAD Salvage Synthesis Pathway, Are of Negative Prognostic Value in Colorectal Cancer.

Frontiers in oncology

Li XQ,Lei J,Mao LH,Wang QL,Xu F,Ran T,Zhou ZH,He S

Tue Sep 29 00:00:00 EDT 2020

Dietary Antioxidant Trans-Cinnamaldehyde Reduced Visfatin-Induced Breast Cancer Progression: In Vivo and In Vitro Study.

Antioxidants (Basel, Switzerland)

Chiang YF,Chen HY,Huang KC,Lin PH,Hsia SM

Fri Dec 06 00:00:00 EST 2019

Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Journal of molecular medicine (Berlin, Germany)

Sun Y,Tian Z,Liu N,Zhang L,Gao Z,Sun X,Yu M,Wu J,Yang F,Zhao Y,Ren H,Chen H,Zhao D,Wang Y,Dong S,Xu C,Lu F,Zhang W

Sun Apr 01 00:00:00 EDT 2018

Exogenous H₂S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Journal of molecular medicine (Berlin, Germany)

Sun Y,Tian Z,Liu N,Zhang L,Gao Z,Sun X,Yu M,Wu J,Yang F,Zhao Y,Ren H,Chen H,Zhao D,Wang Y,Dong S,Xu C,Lu F,Zhang W

Sun Apr 01 00:00:00 EDT 2018

Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing.

British journal of pharmacology

Zhou CC,Yang X,Hua X,Liu J,Fan MB,Li GQ,Song J,Xu TY,Li ZY,Guan YF,Wang P,Miao CY

Mon Aug 01 00:00:00 EDT 2016

Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing.

British journal of pharmacology

Zhou CC,Yang X,Hua X,Liu J,Fan MB,Li GQ,Song J,Xu TY,Li ZY,Guan YF,Wang P,Miao CY

Mon Aug 01 00:00:00 EDT 2016

Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing.

British journal of pharmacology

Zhou CC,Yang X,Hua X,Liu J,Fan MB,Li GQ,Song J,Xu TY,Li ZY,Guan YF,Wang P,Miao CY

Mon Aug 01 00:00:00 EDT 2016

Hepatic NAD(+) deficiency as a therapeutic target for non-alcoholic fatty liver disease in ageing.

British journal of pharmacology

Zhou CC,Yang X,Hua X,Liu J,Fan MB,Li GQ,Song J,Xu TY,Li ZY,Guan YF,Wang P,Miao CY

Mon Aug 01 00:00:00 EDT 2016

NMNAT3 is involved in the protective effect of SIRT3 in Ang II-induced cardiac hypertrophy.

Experimental cell research

Yue Z,Ma Y,You J,Li Z,Ding Y,He P,Lu X,Jiang J,Chen S,Liu P

Sat Oct 01 00:00:00 EDT 2016

NMNAT3 is involved in the protective effect of SIRT3 in Ang II-induced cardiac hypertrophy.

Experimental cell research

Yue Z,Ma Y,You J,Li Z,Ding Y,He P,Lu X,Jiang J,Chen S,Liu P

Sat Oct 01 00:00:00 EDT 2016

Effect of curcumin on visfatin and zinc-α2-glycoprotein in a rat model of non-alcoholic fatty liver disease.

Acta cirurgica brasileira

Li C,Li J,Chen Y,Zhong X,Kang M

Tue Nov 01 00:00:00 EDT 2016

Effect of curcumin on visfatin and zinc-α2-glycoprotein in a rat model of non-alcoholic fatty liver disease.

Acta cirurgica brasileira

Li C,Li J,Chen Y,Zhong X,Kang M

Tue Nov 01 00:00:00 EDT 2016

Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage.

Cancer cell

Tummala KS,Gomes AL,Yilmaz M,Graña O,Bakiri L,Ruppen I,Ximénez-Embún P,Sheshappanavar V,Rodriguez-Justo M,Pisano DG,Wagner EF,Djouder N

Mon Dec 08 00:00:00 EST 2014

Inhibition of de novo NAD(+) synthesis by oncogenic URI causes liver tumorigenesis through DNA damage.

Cancer cell

Tummala KS,Gomes AL,Yilmaz M,Graña O,Bakiri L,Ruppen I,Ximénez-Embún P,Sheshappanavar V,Rodriguez-Justo M,Pisano DG,Wagner EF,Djouder N

Mon Dec 08 00:00:00 EST 2014

Bioinformatics

Protein Aliases: DKFZp666B131; EC 2.4.2.12; MGC117256; NAmPRTase; nampt; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase a; PBEF; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; Visfatin

View more View less

Gene Aliases: 1110035O14Rik; AI314458; AI480535; NAmPRTase; NAMPT; nampta; PBEF; PBEF1; VF; VISFATIN

View more View less

UniProt ID: (Human) P43490, (Mouse) Q99KQ4, (Rat) Q80Z29

View more View less

Entrez Gene ID: (Human) 10135, (Mouse) 59027, (Rat) 297508, (Zebrafish) 100330160

View more View less

Function(s)

nicotinate-nucleotide diphosphorylase (carboxylating) activity cytokine activity protein binding drug binding protein homodimerization activity nicotinamide phosphoribosyltransferase activity transferase activity transferase activity, transferring glycosyl groups catalytic activity transferase

Process(es)

nicotinamide metabolic process signal transduction cell-cell signaling female pregnancy circadian rhythm positive regulation of cell proliferation insulin receptor signaling pathway NAD biosynthetic process response to organic cyclic compound circadian regulation of gene expression positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle cell proliferation positive regulation of nitric-oxide synthase biosynthetic process adipose tissue development pyridine nucleotide biosynthetic process rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

NAMPT/PBEF Polyclonal Antibody

NAMPT/PBEF Antibody (11776-1-AP) in IHC (P)

Product Details

11776-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!