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|Tested species reactivity||Human, Pig|
|Published species reactivity||Pig|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (QNQRMKSKRWQKNN) corresponding to the internal amino acids of NANOG|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||0.03-0.1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
This antibody is predicted to react with canine based on sequence homology.
This antibody has been successfully tested in a Sandwhich ELISA as both a capture and a detection antibody in combination with PA5-18678.
Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Multipotent progenitor cells derived from adult peripheral blood of swine have high neurogenic potential in vitro.
PA5-18406 was used in immunocytochemistry to investigate the differentiation of peripheral blood-derived multipotent adult progenitor cells into neural cells
|Spitzer N,Sammons GS,Butts HM,Grover LM,Price EM||Journal of cellular physiology (226:3156)||2011|
HGNC:20857; homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox