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NANOS3 Polyclonal Antibody
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FIGURE: 1 / 3
NANOS3 Antibody (600-401-CZ1) in ICC/IF
Immunofluorescence Microscopy of Nanos3 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Nanos3 antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Nanos3 as a red fluorescent signal.
Product Details
600-401-CZ1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Nanos3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide near the C-terminus of the human Nanos3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nanos homolog 3; NOS-3
Gene Aliases: NANOS1L; NANOS3; NOS3; ZC2HC12C
UniProt ID: (Human) P60323, (Mouse) P60324
Entrez Gene ID: (Human) 342977, (Mouse) 244551, (Rat) 288909
regulation of translation
germ cell development
spermatogenesis
negative regulation of translation
cell differentiation
oogenesis
regulation of cell cycle
mRNA destabilization
apoptotic signaling pathway
negative regulation of apoptotic signaling pathway
multicellular organism development
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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