Proteintech

NCAM1/CD56 Polyclonal Antibody

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Antibody Testing Data (19)

NCAM1/CD56 Antibody in Western Blot (WB)

FIGURE: 1 / 19

NCAM1/CD56 Antibody (14255-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 14255-1-AP) NCAM1/CD56 antibody at dilution of 1:15,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

NCAM1/CD56 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human small cell lung carcinoma tissue slide using (Product # 14255-1-AP) NCAM1/CD56 antibody at dilution of 1:16,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NCAM1/CD56 Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug NCAM1/CD56 antibody (14255-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

14255-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 21 publications 21 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunocytochemistry (ICC/IF)

View 15 publications 15 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Pig, Rat, Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NCAM1/CD56 Fusion Protein Ag5528 (30-352 aa encoded by BC047244)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: EISVGESKF FLCQVAGDAK DKDISWFSPN GEKLTPNQQR ISVVWNDDSS STLTIYNANI DDAGIYKCVV TGEDGSESEA TVNVKIFQKL MFKNAPTPQE FREGEDAVIV CDVVSSLPPT IIWKHKGRDV ILKKDVRFIV LSNNYLQIRG IKKTDEGTYR CEGRILARGE INFKDIQVIV NVPPTIQARQ NIVNATANLG QSVTLVCDAE GFPEPTMSWT KDGEQIEQEE DDEKYIFSDD SSQLTIKKVD KNDEAEYICI AENKAGEQDA TIHLKVFAKP KITYVENQTA MELEEQVTLT CEASGDPIPS ITWRTSTRNI SSEE (30-352 aa encoded by BC047244)

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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