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Fabgennix
NEDD4 Polyclonal Antibody
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
NEDD4-101AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide taken within amino acid region 840-880 on rat Nedd4 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5-1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HECT-type E3 ubiquitin transferase NEDD4; Nedd4; neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842; ubiquitin protein ligase; unnamed protein product
Gene Aliases: E430025J12Rik; EG639396; Gm7265; KIAA0093; Nedd4-1; Nedd4a
UniProt ID: (Rat) Q62940, (Mouse) P46935
Entrez Gene ID: (Rat) 25489, (Mouse) 17999
ubiquitin-protein transferase activity
protein binding
channel inhibitor activity
potassium channel inhibitor activity
sodium channel inhibitor activity
enzyme binding
protein domain specific binding
beta-2 adrenergic receptor binding
ionotropic glutamate receptor binding
ubiquitin binding
ion channel binding
phosphoserine binding
phosphothreonine binding
ubiquitin protein ligase activity
RNA polymerase binding
proline-rich region binding
negative regulation of transcription from RNA polymerase II promoter
adaptive immune response
outflow tract morphogenesis
endocardial cushion development
ubiquitin-dependent protein catabolic process
protein monoubiquitination
protein targeting to lysosome
immune response
cellular response to DNA damage stimulus
lysosomal transport
neuromuscular junction development
regulation of cell communication
negative regulation of sodium ion transport
response to denervation involved in regulation of muscle adaptation
protein ubiquitination
regulation of signaling
negative regulation of vascular endothelial growth factor receptor signaling pathway
neuron projection development
receptor internalization
receptor catabolic process
cellular response to UV
T cell activation
regulation of protein catabolic process
regulation of membrane potential
glucocorticoid receptor signaling pathway
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
positive regulation of protein catabolic process
positive regulation of nucleocytoplasmic transport
blood vessel morphogenesis
regulation of dendrite morphogenesis
regulation of synapse organization
progesterone receptor signaling pathway
regulation of transport
positive regulation of protein kinase B signaling
protein K63-linked ubiquitination
regulation of postsynaptic neurotransmitter receptor internalization
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
regulation protein catabolic process at postsynapse
negative regulation of potassium ion export across plasma membrane
transcription from RNA polymerase II promoter
development involved in symbiotic interaction
innate immune response
viral budding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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