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NEDD4 Polyclonal Antibody
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Product Details
OSN00008W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from the WW1 domain of mouse NEDD4 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The antigen shares 96% identity with human NEDD4.
Specificity of this antibody: NEDD4.
Target Information
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase NEDD4; HECT-type E3 ubiquitin transferase NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4a; neural precursor cell expressed, developmentally down-regulted gene 4; OTTHUMP00000217840; OTTHUMP00000217841; OTTHUMP00000217842
Gene Aliases: AA959633; AL023035; AU019897; E430025J12Rik; Kiaa0093; mKIAA0093; Nedd-4; Nedd4; Nedd4-1; Nedd4a; Rpf1
UniProt ID: (Mouse) P46935
Entrez Gene ID: (Mouse) 17999
ubiquitin-protein transferase activity
protein binding
protein C-terminus binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
sodium channel inhibitor activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
phosphoserine binding
phosphothreonine binding
ubiquitin protein ligase activity
proline-rich region binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
adaptive immune response
outflow tract morphogenesis
endocardial cushion development
protein monoubiquitination
nervous system development
neuromuscular junction development
negative regulation of sodium ion transport
protein ubiquitination
negative regulation of vascular endothelial growth factor receptor signaling pathway
T cell activation
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of protein catabolic process
blood vessel morphogenesis
regulation of dendrite morphogenesis
regulation of synapse organization
protein K63-linked ubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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