Immunoflourescent analysis of NEFH in mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody and Neurofilament Heavy (NF-H) antibody (Product # PA1-32434). DNA staining is shown in blue. The antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.
|Tested species reactivity||Bovine, Cat, Human, Mouse, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Bovine NF-H protein filaments prepared from spinal cords by the glycerol polymerization method, and dissolved in 6M urea.|
|Contains||5mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-32434 detects NEFH from feline, human, mouse, rat samples.
PA1-32434 has been successfully used in immunofluorescence, Western blot applications.
The PA1-32434 immunogen is: Bovine NF-H protein filaments prepared from spinal cords by the glycerol polymerization method, and dissolved in 6M urea.
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.