For Western blot using alkaline phosphatase plus chromagen, a dilution of at least 1:1000 is recommended. For chemiluminescence, use at a dilution of 1:20,000.| Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
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Protein Aliases: 68 kDa neurofilament protein; 70 kDa neurofilament protein; CMT1F; CMT2E; light molecular weight neurofilament protein; Micro glutamic acid-rich protein; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L pro; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF-L; NFL; NLC; protein phosphatase 1, regulatory subunit 110
Gene Aliases: AI847934; BOS_9100; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110