Proteintech

NELFE Polyclonal Antibody

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NELFE Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10705-1-AP (NELFE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NELFE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 10705-1-AP (NELFE antibody) at dilution of 1:100 (under 40x lens).

NELFE Antibody in Western Blot (WB) — Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

NELFE Antibody in Immunoprecipitation (IP) — IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:800) with mouse brain tissue lysate 4680ug.

Product Details

10705-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:12,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
ChIP assay (ChIP)	Assay-dependent	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NELFE Fusion Protein Ag1186 (1-333 aa encoded by BC011600) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

10705-1-AP is obtained by immunizing animals with full-length RDBP of human origin.

Immunogen sequence: MLVIPPGLS EEEEALQKKF NKLKKKKKAL LALKKQSSSS TTSQGGVKRS LSEQPVMDTA TATEQAKQLV KSGAISAIKA ETKNSGFKRS RTLEGKLKDP EKGPVPTFQP FQRSISADDD LQESSRRPQR KSLYESDRLR ELGPDGEEAE GPGAGDGPPR SFDWGYEERS GAHSSASPPR SRSRDRSHER NRDRDREGPF RRSDSFPERR APRKGNTLYV YGEDMTPTLL RGAFSPFGNI IDLSMDPPRN CAFVTYEKME SADQAVAELN GTQVESVQLK VNIARKQPML DAATGKSVWG SLAVQNSPKG CHRDKRTQIV YSDDVYKENL VDGF (1-333 aa encoded by BC011600)

Target Information

RDBP is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 EST 2019

Negative elongation factor is essential for endometrial function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hewitt SC,Li R,Adams N,Winuthayanon W,Hamilton KJ,Donoghue LJ,Lierz SL,Garcia M,Lydon JP,DeMayo FJ,Adelman K,Korach KS

Fri Feb 01 00:00:00 EST 2019

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 EST 2018

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

eLife

Sacta MA,Tharmalingam B,Coppo M,Rollins DA,Deochand DK,Benjamin B,Yu L,Zhang B,Hu X,Li R,Chinenov Y,Rogatsky I

Fri Feb 09 00:00:00 EST 2018

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Nucleic acids research

Lu X,Zhu X,Li Y,Liu M,Yu B,Wang Y,Rao M,Yang H,Zhou K,Wang Y,Chen Y,Chen M,Zhuang S,Chen LF,Liu R,Chen R

Fri Aug 19 00:00:00 EDT 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 EST 2016

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Neurobiology of disease

Bastide MF,Bido S,Duteil N,Bézard E

Fri Jan 01 00:00:00 EST 2016

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 EDT 2013

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Proceedings of the National Academy of Sciences of the United States of America

Gupte R,Muse GW,Chinenov Y,Adelman K,Rogatsky I

Tue Sep 03 00:00:00 EDT 2013

Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.

Oncology reports

Iida M,Iizuka N,Tsunedomi R,Tsutsui M,Yoshida S,Maeda Y,Tokuhisa Y,Sakamoto K,Yoshimura K,Tamesa T,Oka M

Wed Aug 01 00:00:00 EDT 2012

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Cell

Sawarkar R,Sievers C,Paro R

Fri May 11 00:00:00 EDT 2012

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Cell

Sawarkar R,Sievers C,Paro R

Fri May 11 00:00:00 EDT 2012

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 EDT 2009

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Proceedings of the National Academy of Sciences of the United States of America

Adelman K,Kennedy MA,Nechaev S,Gilchrist DA,Muse GW,Chinenov Y,Rogatsky I

Tue Oct 27 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: major histocompatibility complex gene RD; Negative elongation factor E; negative elongation factor polypeptide E; NELF E; NELF-E; nuclear protein; RD RNA binding protein; RD RNA-binding protein; RNA-binding protein RD

Gene Aliases: D17H6S45; D6S45; NELF-E; NELFE; RD; RDBP; RDP

UniProt ID: (Human) P18615, (Mouse) P19426

Entrez Gene ID: (Human) 7936, (Mouse) 27632, (Rat) 294258

Function(s)