MA3-024 detects NFATc1 (NFAT2) from transfected human, rat, mouse, and monkey cells. This antibody does not cross react with NFATc2 (NFAT1).
MA3-024 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluorescence and gel shift procedures. By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT2 from COS cell lysate.
The MA3-024 immunogen is bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: calcineurin-dependent 1; cytoplasmic; MGC138448; NF-ATC; NF-ATc1; NFAT trans; NFAT transcription complex cytosolic component; NFAT2; NFATC; NFATC1; nuclear factor of activated T-cells; nuclear factor of activated T-cells 'c'; Nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; transcription factor NF-ATc
Gene Aliases: 2210017P03Rik; AI449492; AV076380; NF-ATC; NF-ATc1.2; NFAT2; NFATC; NFATC1; Nfatcb
UniProt ID: (Human) O95644
Molecular Function: transcription factor