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Bethyl Laboratories
NFIB Polyclonal Antibody
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FIGURE: 1 / 1
NFIB Antibody (A303-565A) in WB
Detection of human NFIB by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NFIB antibody (Product # A303-565A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. NFIB was also immunoprecipitated by rabbit anti-NFIB antibody A303-566A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Product Details
A303-565A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 225 and 275 of human Nuclear Factor I/B.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Bovine and Golden hamster
Target Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; Nuclear factor 1 B-type; nuclear factor 1/B; Nuclear factor I/B; TGGCA-binding protein; transcription factor; unnamed protein product
Gene Aliases: CTF; HMGIC/NFIB; MACID; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB; NFIB2; NFIB3
UniProt ID: (Human) O00712
Entrez Gene ID: (Human) 4781
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription regulator inhibitor activity
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
tissue homeostasis
chondrocyte differentiation
glandular epithelial cell differentiation
DNA replication
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
brain development
cell proliferation
response to wounding
response to bacterium
glial cell differentiation
exit from mitosis
gene expression
glial cell proliferation
stem cell population maintenance
principal sensory nucleus of trigeminal nerve development
glial cell fate specification
cell proliferation in forebrain
anterior commissure morphogenesis
neurogenesis
lung development
forebrain development
hindbrain development
regeneration
negative regulation of DNA binding
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
neuron fate specification
generation of neurons
retina development in camera-type eye
Clara cell differentiation
Type I pneumocyte differentiation
Type II pneumocyte differentiation
salivary gland cavitation
cell differentiation involved in salivary gland development
lung ciliated cell differentiation
neural precursor cell proliferation
commissural neuron axon guidance
stem cell proliferation
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of stem cell proliferation
negative regulation of mesenchymal cell proliferation involved in lung development
negative regulation of epithelial cell proliferation involved in lung morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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