Immunofluorescent analysis of NFkB p65 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an NFkB p65 polyclonal antibody (Product # PA1-30408) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide EDFSSIADMDFSALLSQISS, corresponding to the C-terminus of Human NFkB p65.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30408 detects NFkB p65 from rat, mouse, human samples.
PA1-30408 has been successfully used in immunohistochemistry (paraffin tissue), immunofluorescence, immunoprecipitation, Western blot applications.
The PA1-30408 immunogen is Synthetic peptide corresponding to the C-terminus of Human NFkB p65.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.