Immunofluorescent analysis of NFkB p65 showing staining in the cytoplasm of U-251 cells using a NFkB p65 polyclonal antibody (Product # PA5-35232) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 166-195 amino acids from the central region of human RELA|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex.The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form ofNFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA,MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB bytrapping it in the cytoplasm. Phosphorylation of serine residues onthe I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM603258) marks them for destruction via the ubiquitination pathway,thereby allowing activation of the NFKB complex. Activated NFKBcomplex translocates into the nucleus and binds DNA atkappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or Gpurine; and Y is a C or T pyrimidine).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.