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Invitrogen
NGAL Polyclonal Antibody
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FIGURE: 1 / 6
NGAL Antibody (PA5-79589) in ICC/IF
Immunofluorescence analysis of NGAL was performed using 70% confluent log phase A549 treated with Thapsigargin (500nM, 48hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NGAL Polyclonal Antibody (Product # PA5-79589) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor... View More
Product Details
PA5-79589
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Lipocalin 2 recombinant protein (Position: Q21-G198).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A549 whole cell, SMMC whole cell. IHC: human intestinal cancer tissue.
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HGNC:6526; LCN2; Lipocalin-2; Lipocalin2; migration-stimulating factor inhibitor; Neutrophil gelatinase-associated lipocalin; neutrophil lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; Siderocalin; siderocalin LCN2; unnamed protein product; uterocalin
Gene Aliases: 24p3; HNL; LCN2; MSFI; NGAL; p25
UniProt ID: (Human) P80188
Entrez Gene ID: (Human) 3934
immune system process
ion transport
iron ion transport
apoptotic process
acute-phase response
response to oxidative stress
short-term memory
long-term memory
response to xenobiotic stimulus
response to virus
response to bacterium
response to herbicide
response to toxic substance
response to blue light
response to fructose
response to iron(II) ion
response to mycotoxin
positive regulation of endothelial cell migration
positive regulation of gene expression
siderophore transport
positive regulation of cell projection organization
response to nutrient levels
cellular response to nutrient levels
response to lipopolysaccharide
cellular response to increased oxygen levels
defense response to bacterium
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
innate immune response
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to X-ray
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of hippocampal neuron apoptotic process
negative regulation of hippocampal neuron apoptotic process
positive regulation of cold-induced thermogenesis
positive regulation of reactive oxygen species biosynthetic process
response to kainic acid
positive regulation of iron ion import across plasma membrane
cellular response to beta-amyloid
positive regulation of endothelial tube morphogenesis
cellular response to nerve growth factor stimulus
positive regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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