Proteintech

NIT1 Polyclonal Antibody

Product Image Gallery

NIT1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NIT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens).

NIT1 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Western Blot (WB)

1:500-1:1000

Published Applications

Immunohistochemistry (IHC)

See 1 publication below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NIT1 Fusion Protein Ag5764

Conjugate

Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.