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NME2 Antibody (PA5-79744) in ICC/IF
Immunocytochemistry analysis of NME2 using anti-NME2 antibody (Product # PA5-79744). NME2 was detected in a section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NME2 antibody (Product # PA5-79744)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visuali... View More
Product Details
PA5-79744
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NME2 (137-152aa EELVDYKSCAHDWVYE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746859
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue. IHC: Human Intestinal Cancer Tissue, Rat Cerebellum Tissue, mouse intestine tissue. ICC/IF: U20S cell, HELA Cell. Flow: HL-60 cell.
Target Information
Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.13.3; C-myc purine-binding transcription factor PUF; c-myc transcription factor; epididymis secretory sperm binding protein Li 155an; expressed in non-metastatic cells 2; expressed in non-metastatic cells 2 protein; HEL-S-155an; Histidine protein kinase NDKB; NDK B; NDP kinase alpha; NDP kinase B; nm23-H2; nm23-M2; non-metastatic cells 2, protein (NM23) expressed in; non-metastatic cells 2, protein (NM23B) expressed in; Nucleoside diphosphate kinase B; nucleoside-diphosphate kinase 2; nucleotide diphosphate kinase B; P18
Gene Aliases: NDKB; NDPK-B; NDPKB; nm23-2; NM23-H2; nm23-M2; NM23B; NME2; p18-12d; PUF
UniProt ID: (Human) P22392, (Rat) P19804, (Mouse) Q01768
Entrez Gene ID: (Human) 4831, (Rat) 83782, (Mouse) 18103
DNA binding
transcription factor activity, sequence-specific DNA binding
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein binding
ATP binding
metal ion binding
protein serine/threonine kinase activity
fatty acid binding
drug binding
intermediate filament binding
enzyme binding
nucleotide binding
kinase activity
transferase activity
kinase
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
transcription, DNA-templated
regulation of transcription, DNA-templated
cell adhesion
integrin-mediated signaling pathway
nucleoside triphosphate biosynthetic process
nucleobase-containing small molecule interconversion
peptidyl-histidine phosphorylation
negative regulation of apoptotic process
positive regulation of keratinocyte differentiation
regulation of epidermis development
positive regulation of transcription from RNA polymerase II promoter
positive regulation of epithelial cell proliferation
mitophagy in response to mitochondrial depolarization
negative regulation of myeloid leukocyte differentiation
positive regulation of neuron projection development
positive regulation of cAMP biosynthetic process
cellular response to oxidative stress
regulation of apoptotic process
protein autophosphorylation
protein oligomerization
response to growth hormone
cellular response to glucose stimulus
cellular response to fatty acid
nucleotide metabolic process
phosphorylation
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