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Invitrogen
NOD1 Polyclonal Antibody
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FIGURE: 1 / 6
NOD1 Antibody (PA5-79747) in ICC/IF
Immunocytochemistry analysis of NOD1 on SMMC-7721 cells. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with NOD1 polyclonal antibody (Product# PA5-79747) with a dilution of 1 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Product Details
PA5-79747
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CARD4 recombinant protein (Position: M1-M160).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A549 whole cell, Rat Cardiac Muscle Tissue. IHC: Human Placenta tissue. ICC/IF: A549 Cell, Hela Cell, SMMC-7721 Cell.
Target Information
NOD1 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD1 contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and ten tandem leucine-rich repeats (LRRs) in its C-terminus. The CARD is involved in apoptotic signaling, and NOD1 activates caspase-9 and NF-kappa-B. LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apaf-1/Ced-4 family member; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CED-4/APAF-1 cell death family member; hNod1; NLR family, CARD domain containing 1; NOD1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1; unnamed protein product
Gene Aliases: CARD4; CLR7.1; hNod1; NLRC1; NOD1; RGD1562269
UniProt ID: (Human) Q9Y239
Entrez Gene ID: (Human) 10392, (Rat) 500133
nucleotide binding
protein binding
ATP binding
cysteine-type endopeptidase activator activity involved in apoptotic process
pattern recognition receptor activity
identical protein binding
protein homodimerization activity
peptidoglycan binding
ubiquitin binding
macromolecular complex binding
CARD domain binding
scaffold/adaptor protein
positive regulation of cytokine production
pattern recognition receptor signaling pathway
immune system process
positive regulation of dendritic cell antigen processing and presentation
apoptotic process
defense response
inflammatory response
signal transduction
JNK cascade
detection of biotic stimulus
positive regulation of autophagy
detection of bacterium
positive regulation of interleukin-1 beta production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of stress-activated MAPK cascade
response to endoplasmic reticulum stress
intracellular signal transduction
defense response to bacterium
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of JNK cascade
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
stress-activated MAPK cascade
positive regulation of macrophage cytokine production
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
nucleotide-binding oligomerization domain containing 1 signaling pathway
cellular response to muramyl dipeptide
xenophagy
positive regulation of NIK/NF-kappaB signaling
positive regulation of xenophagy
activation of cysteine-type endopeptidase activity involved in apoptotic process
interleukin-8 biosynthetic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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