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Invitrogen
NOD1 Polyclonal Antibody
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FIGURE: 1 / 6
NOD1 Antibody (PA5-79747) in ICC/IF
Immunocytochemistry analysis of NOD1 on SMMC-7721 cells. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with NOD1 polyclonal antibody (Product# PA5-79747) with a dilution of 1 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Product Details
PA5-79747
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CARD4 recombinant protein (Position: M1-M160).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: A549 whole cell, Rat Cardiac Muscle Tissue. IHC: Human Placenta tissue. ICC/IF: A549 Cell, Hela Cell, SMMC-7721 Cell.
Target Information
NOD1 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD1 contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and ten tandem leucine-rich repeats (LRRs) in its C-terminus. The CARD is involved in apoptotic signaling, and NOD1 activates caspase-9 and NF-kappa-B. LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; hNod1; NLR family, CARD domain containing 1; nucleotide-binding oligomerization domain containing 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1
Gene Aliases: CARD4; CLR7.1; NLRC1; NOD1; RGD1562269
UniProt ID: (Human) Q9Y239
Entrez Gene ID: (Human) 10392, (Rat) 500133
activation of MAPK activity
positive regulation of dendritic cell antigen processing and presentation
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
defense response
inflammatory response
signal transduction
JNK cascade
detection of biotic stimulus
detection of bacterium
positive regulation of interleukin-1 beta production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
intracellular signal transduction
interleukin-8 biosynthetic process
defense response to bacterium
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
innate immune response
positive regulation of JNK cascade
defense response to Gram-positive bacterium
positive regulation of nitric-oxide synthase activity
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
positive regulation of ERK1 and ERK2 cascade
nucleotide-binding oligomerization domain containing signaling pathway
cellular response to muramyl dipeptide
positive regulation of NIK/NF-kappaB signaling
positive regulation of xenophagy
positive regulation of stress-activated MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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