Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
NOL3 Fusion Protein Ag1306 (1-208 aa encoded by BC012798) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.53 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MGNAQERPS ETIDRERKRL VETLQADSGL LLDALLARGV LTGPEYEALD ALPDAERRVR RLLLLVQGKG EAACQELLRC AQRTAGAPDP AWDWQHVGPG YRDRSYDPPC PGHWTPEAPG SGTTCPGLPR ASDPDEAGGP EGSEAVQSGT PEEPEPELEA EASKEAEPEP EPEPELEPEA EAEPEPELEP EPDPEPEPDF EERDESEDS (1-208 aa encoded by BC012798 )
ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis repressor with CARD; Muscle-enriched cytoplasmic protein; Myp; Nop 30; Nop30; Nucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa
Gene Aliases: ARC; B430311C09Rik; FCM; MYC; MYP; NOL3; NOP; NOP30
UniProt ID: (Human) O60936, (Rat) Q62881, (Mouse) Q9D1X0
Entrez Gene ID: (Human) 8996, (Rat) 85383, (Mouse) 78688
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