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Invitrogen
NOLA1 Polyclonal Antibody
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FIGURE: 1 / 11
NOLA1 Antibody (PA5-143962) in ICC/IF
Immunocytochemistry analysis of NOLA1 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in NOLA1 Polyclonal antibody (Product # PA5-143962) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate ... View More
Product Details
PA5-143962
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NOLA1/GAR1 recombinant protein (Position: F58-K165).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A375 whole cell, human K562 whole cell, human HL-60 whole cell, human MCF-7 whole cell. IHC: human breast cancer tissue, human gastric carcinoma tissue, human liver cancer tissue, human ovarian adenoma tissue, human pancreatic cancer tissue, human prostate cancer tissue, human glioma tissue, human lymphoma tissue. ICC/IF: MCF-7 cell. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Required for ribosome biogenesis and telomere maintenance. NOLA1 is part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. NOLA1 may also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GAR1 homolog, ribonucleoprotein; GAR1 ribonucleoprotein homolog; H/ACA ribonucleoprotein complex subunit 1; Nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs); snoRNP protein GAR1
Gene Aliases: GAR1; NOLA1
UniProt ID: (Human) Q9NY12
Entrez Gene ID: (Human) 54433
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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