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Invitrogen
NOTCH2 (Cleaved Ala1734) Polyclonal Antibody
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FIGURE: 1 / 3
NOTCH2 (Cleaved Ala1734) Antibody (PA5-99429) in IHC (P)
Immunohistochemistry analysis of NOTCH2 (Cleaved Ala1734) in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with NOTCH2 (Cleaved Ala1734) polyclonal antibody (Product # PA5-99429) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99429
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NOTCH2(Accession Q04721), corresponding to amino acid residues N1723-T1752.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of fragment of activated Notch2 resulting from cleavage adjacent to Ala1734.
Target Information
The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four Notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The Notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: membrane bound receptor; Motch B; Neurogenic locus notch homolog protein 2; Notch 2; Notch gene homolog 2; Notch homolog 2; putative cell-fate determining protein; transmembrane protein; unnamed protein product
Gene Aliases: AGS2; AI853703; HJCYS; hN2; N2; NOTCH2
UniProt ID: (Human) Q04721, (Mouse) O35516
Entrez Gene ID: (Human) 4853, (Mouse) 18129, (Rat) 29492
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
cell fate determination
liver development
placenta development
heart looping
morphogenesis of an epithelial sheet
marginal zone B cell differentiation
inflammatory response to antigenic stimulus
atrioventricular node development
pulmonary valve morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
humoral immune response
Notch signaling pathway
determination of left/right symmetry
nervous system development
axon guidance
cell proliferation
positive regulation of cell proliferation
organ morphogenesis
negative regulation of macromolecule biosynthetic process
negative regulation of gene expression
positive regulation of keratinocyte proliferation
hemopoiesis
cell differentiation
embryonic limb morphogenesis
BMP signaling pathway
positive regulation of BMP signaling pathway
multicellular organism growth
intracellular signal transduction
intrahepatic bile duct development
wound healing
defense response to bacterium
regulation of apoptotic process
myeloid dendritic cell differentiation
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of Ras protein signal transduction
bone remodeling
regulation of developmental process
positive regulation of smooth muscle cell differentiation
cardiac septum morphogenesis
atrial septum morphogenesis
placenta blood vessel development
ciliary body morphogenesis
positive regulation of ERK1 and ERK2 cascade
left/right axis specification
cellular response to tumor cell
proximal tubule development
glomerular visceral epithelial cell development
glomerular capillary formation
hepatocyte proliferation
liver morphogenesis
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of hemopoiesis
cholangiocyte proliferation
regulation of osteoclast development
transcription, DNA-templated
cell cycle arrest
multicellular organism development
negative regulation of cell proliferation
cell growth
interleukin-4 secretion
tissue regeneration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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