Invitrogen

NOX1 Polyclonal Antibody

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NOX1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NOX1 in HepG2 cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with NOX1 polyclonal antibody (Product # PA5-103220) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

NOX1 Antibody in Western Blot (WB) — Western blot analysis of NOX1 in mouse kidney and rat brain. Samples were incubated with NOX1 polyclonal antibody (Product # PA5-103220).

NOX1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — FIGURE 1 The protein levels of NOX1 and NOX4 were increased by DOX both in vivo and in vitro . (A) Representative western blot analysis of NOX1 and NOX4 in myocardial tissue. (B,C) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 6 per group). (D,E) Representative images of NOX1 and NOX4 expression in myocardial tissue detected by the immunofluorescence. The nuclei were stained with DAPI (Scale bar = 50 um). (F) Representative western blot analysis of NOX1 and NOX4 of NRCMs treated with DOX of different concentrations for 24 h (G,H) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 3). (I) Representative western blot analysis of NOX 1 and NOX 4 of NRCMs treated with DOX for different time. (J,K) Quantification of NOX1 and NOX4 expression relative to the beta-actin level ( n = 4-5). * p < 0.05, ** p < 0.01, *** p < 0.001, n. s., not significant. NOX, NADPH oxidase; DOX, doxorubicin; cTnI, Cardiac troponin I.

Product Details

PA5-103220

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human NOX1, corresponding to a region within C-terminal amino acids. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2852587

Target Information

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H (+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. NOX1 have the potential to be effective treatments for a range of ischemic diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.

Frontiers in pharmacology

Zheng H,Xu N,Zhang Z,Wang F,Xiao J,Ji X

Published figure using NOX1 polyclonal antibody (Product # PA5-103220) in Immunohistochemistry

Sat Jul 16 00:00:00 EDT 2022

Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway.

Frontiers in pharmacology

Zheng H,Xu N,Zhang Z,Wang F,Xiao J,Ji X

Published figure using NOX1 polyclonal antibody (Product # PA5-103220) in Immunohistochemistry

Sat Jul 16 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: GP91 phox homolog; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); Mitogenic oxidase 1; MOX-1; MOX1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase 1 alpha; NADPH oxidase homolog-1; NOH-1; NOX-1

Gene Aliases: GP91-2; MOX1; NOH-1; NOH1; Nox-1; NOX1; NOX1a; NOX1alpha

UniProt ID: (Human) Q9Y5S8, (Rat) Q9WV87, (Mouse) Q8CIZ9

Entrez Gene ID: (Human) 27035, (Rat) 114243, (Mouse) 237038

Molecular Function: oxidase

Function(s)

Process(es)

protein binding superoxide-generating NADPH oxidase activity voltage-gated proton channel activity metal ion binding Rac GTPase binding NADP binding N-ethylmaleimide reductase activity oxidoreductase activity reduced coenzyme F420 dehydrogenase activity sulfur oxygenase reductase activity malolactic enzyme activity NADPH:sulfur oxidoreductase activity epoxyqueuosine reductase activity

NOX1 Polyclonal Antibody

Product Details

PA5-103220

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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