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NPAS4 Antibody (MA5-45749) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with NPAS4 monoclonal antibody (Product # MA5-45749) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C... View More
Product Details
MA5-45749
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N408/79
Immunogen
Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932203
Product Specific Information
A 1:100 dilution of MA5-45749 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 90kDa.approximately
This antibody was formerly sold as clone S408-79.
Target Information
NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLH PAS type transcription factor NXF; bHLH-PAS type transcription factor NXF; bHLHe79; Class E basic helix-loop-helix protein 79; HLH-PAS transcription factor NXF; LE-PAS; Limbic-enhanced PAS protein; Neuronal PAS domain-containing protein 4; Neuronal PAS4; PAS domain-containing protein 10; PASD10 and LE-PAS
Gene Aliases: BHLHE79; LE-PAS; NPAS4; NXF; PASD10
UniProt ID: (Human) Q8IUM7, (Rat) Q8CJH6, (Mouse) Q8BGD7
Entrez Gene ID: (Human) 266743, (Rat) 266734, (Mouse) 225872
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein heterodimerization activity
RNA polymerase II regulatory region DNA binding
DNA binding
signal transducer activity
RNA polymerase II transcription factor activity, sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
inhibitory postsynaptic potential
cellular response to corticosterone stimulus
transcription, DNA-templated
signal transduction
positive regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to stress
cellular response to acid chemical
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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