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Product Details
PA5-103956
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:3,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthesized peptide derived from human NPHP1(Accession O15259), corresponding to amino acid residues L468-C488. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2853288 |
Target Information
Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Juvenile nephronophthisis 1 protein; nephrocystin 1; Nephrocystin-1; Nephrocystin1; nephronophthisis 1 (juvenile)
Gene Aliases: JBTS4; NPH1; NPHP1; SLSN1
UniProt ID: (Human) O15259, (Mouse) Q9QY53
Entrez Gene ID: (Human) 4867, (Mouse) 53885
Molecular Function:
cell junction protein
signal transduction
excretion
visual behavior
single organismal cell-cell adhesion
cell projection organization
actin cytoskeleton organization
spermatid differentiation
retina development in camera-type eye
positive regulation of bicellular tight junction assembly
spermatogenesis
cell differentiation
cellular protein localization
photoreceptor cell outer segment organization
Performance Guarantee
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